RINT-1 CRISPR/Cas9 KO Plasmid (h): sc-407428
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RINT-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RINT-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RINT-1 CRISPR/Cas9 KO Plasmid (h) | sc-407428 | 20 µg | $397.00 |
Overview
RINT1 encodes RINT-1, a coiled-coil protein that contributes to Golgi and endoplasmic reticulum (ER) homeostasis by supporting vesicle tethering and membrane trafficking between these compartments. RINT-1 is implicated in maintaining proper secretory pathway organization and in coordinating cellular responses to proteotoxic and replication-associated stress, processes that intersect with genome stability and cell-cycle control. Disruption of RINT1-dependent trafficking can perturb protein processing and organelle integrity, leading to altered signaling and stress adaptation. Genetic and functional studies have linked RINT1 to cancer susceptibility and other proliferative disorders, making it relevant for investigating mechanisms that couple intracellular transport with oncogenic stress responses.
RINT-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RINT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RINT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RINT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RINT-1 protein expression.
This CRISPR knockout system enables efficient generation of RINT1-deficient cell models for investigation of RINT-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RINT1 exon(s) critical for RINT-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RINT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RINT-1 CRISPR/Cas9 KO Plasmid (h) and RINT-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RINT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RINT-1 HDR Plasmid (h) and RINT-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RINT1 homology arms to support homology-directed repair at defined RINT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.