RING1 Double Nickase Plasmid (h): sc-405198-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RING1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RING1 Double Nickase Plasmid (h) and RING1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RING1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RING1 Antibody (8C12F4): sc-517221
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RING1 Double Nickase Plasmid (h) | sc-405198-NIC | 20 µg | $410.00 | |||
RING1 Double Nickase Plasmid (h2) | sc-405198-NIC-2 | 20 µg | $410.00 |
RING1 encodes RING finger protein 1, a core catalytic component of Polycomb repressive complex 1 (PRC1) that mediates monoubiquitination of histone H2A at Lys119 and promotes chromatin compaction. Through PRC1-dependent epigenetic silencing, RING1 helps control developmental gene programs, lineage commitment, and maintenance of transcriptional repression at Polycomb target loci. RING1 interfaces with Polycomb signaling networks and coordinates with PRC2-driven H3K27 trimethylation to stabilize repressive chromatin states. Dysregulated RING1/PRC1 activity is implicated in aberrant transcriptional programs observed in cancer and developmental disorders, supporting its use in studies of chromatin regulation and disease-associated epigenetics.
RING1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RING1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RING1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RING1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RING1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.