RIM-BP2 CRISPR/Cas9 KO Plasmid (h): sc-406740
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RIM-BP2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RIM-BP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RIM-BP2 CRISPR/Cas9 KO Plasmid (h) | sc-406740 | 20 µg | $397.00 |
Overview
RIMBP2 encodes RIM-BP2, a presynaptic active zone scaffold that couples voltage-gated Ca2+ channels to the neurotransmitter release machinery through interactions with RIM proteins and other synaptic components. By organizing Ca2+ influx with synaptic vesicle docking and priming, RIM-BP2 supports efficient action potential–evoked exocytosis and short-term synaptic plasticity at excitatory synapses. This protein participates in Ca2+-dependent signaling at the presynaptic terminal and influences vesicle cycling dynamics that shape synaptic strength. Genetic perturbation of RIMBP2 has been associated with neurodevelopmental and neuropsychiatric phenotypes, making it relevant for studying synaptic transmission defects and circuit-level dysfunction.
RIM-BP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RIMBP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RIMBP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RIMBP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RIM-BP2 protein expression.
This CRISPR knockout system enables efficient generation of RIMBP2-deficient cell models for investigation of RIM-BP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RIMBP2 exon(s) critical for RIM-BP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RIMBP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RIM-BP2 CRISPR/Cas9 KO Plasmid (h) and RIM-BP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RIMBP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RIM-BP2 HDR Plasmid (h) and RIM-BP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RIMBP2 homology arms to support homology-directed repair at defined RIMBP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.