Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RICK Double Nickase Plasmid (h): sc-400731-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RICK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RICK Double Nickase Plasmid (h) and RICK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RIPK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RICK Antibody (A-10): sc-166765
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RICK Double Nickase Plasmid (h) | sc-400731-NIC | 20 µg | $410.00 | |||
RICK Double Nickase Plasmid (h2) | sc-400731-NIC-2 | 20 µg | $410.00 |
RIPK2 encodes receptor-interacting serine/threonine-protein kinase 2 (RICK), a cytosolic kinase that functions as a key adaptor downstream of NOD1 and NOD2 pattern-recognition receptors. Upon sensing bacterial peptidoglycan fragments, RICK promotes polyubiquitination-dependent assembly of signaling complexes that activate NF-κB and MAPK pathways, driving inflammatory gene expression and innate immune responses. RICK also interfaces with ubiquitin signaling networks and can influence cell stress responses and programmed cell death signaling in a context-dependent manner. Dysregulated RIPK2 signaling has been linked to inflammatory and immune-mediated disease biology, including pathways implicated in intestinal inflammation and other chronic inflammatory states.
RICK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RIPK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RIPK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RIPK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RIPK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.