Ribosomal Protein L30 CRISPR/Cas9 KO Plasmid (h): sc-406356

Overview

RPL30 encodes ribosomal protein L30, a core component of the 60S large ribosomal subunit that contributes to ribosome assembly and translational elongation. As part of the ribosome biogenesis and protein synthesis network, RPL30 supports proteostasis and growth-related signaling by enabling efficient mRNA translation. Perturbation of ribosomal proteins can engage nucleolar stress responses and alter p53-dependent checkpoints, linking ribosome function to cell-cycle control and apoptosis. Dysregulated ribosome biogenesis and translational control are recurrent features across proliferative and stress-adaptive states, making RPL30 relevant for studying mechanisms that couple translation capacity to cellular fitness.

Ribosomal Protein L30 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RPL30 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RPL30 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RPL30 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ribosomal Protein L30 protein expression.

This CRISPR knockout system enables efficient generation of RPL30-deficient cell models for investigation of Ribosomal Protein L30 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting RPL30 exon(s) critical for Ribosomal Protein L30 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple RPL30 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Ribosomal Protein L30 CRISPR/Cas9 KO Plasmid (h) and Ribosomal Protein L30 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RPL30 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Ribosomal Protein L30 HDR Plasmid (h) and Ribosomal Protein L30 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RPL30 homology arms to support homology-directed repair at defined RPL30 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.