RhoGEF p115/Lsc Antibody (B-7): sc-373720

RhoGEF p115/Lsc Antibody (B-7) is a mouse monoclonal IgG1 antibody that detects RhoGEF p115/Lsc in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RhoGEF p115/Lsc plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor (GEF) specifically for Rho family GTPases, which are essential for regulating various cellular processes, including cytoskeletal dynamics and cell migration. Anti-RhoGEF p115/Lsc antibody (B-7) selectively recognizes RhoGEF p115/Lsc′s activation of Rho GTPases, while not affecting Rac or Cdc42, highlighting RhoGEF p115/Lsc′s importance in maintaining signaling pathway specificity that governs cell shape and movement. This specificity is vital for processes such as wound healing and immune responses, where precise control of cell behavior is necessary. RhoGEF p115/Lsc (B-7) antibody serves as an invaluable tool for researchers studying cell signaling mechanisms and Rho GTPase activation′s functional implications in health and disease.

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RhoGEF p115/Lsc Antibody (B-7) References:

1. Identification of potential mechanisms for regulation of p115 RhoGEF through analysis of endogenous and mutant forms of the exchange factor.  |  Wells, CD., et al. 2001. J Biol Chem. 276: 28897-905. PMID: 11384980
2. A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin.  |  Dubash, AD., et al. 2007. J Cell Sci. 120: 3989-98. PMID: 17971419
3. Reversible translocation of p115-RhoGEF by G(12/13)-coupled receptors.  |  Meyer, BH., et al. 2008. J Cell Biochem. 104: 1660-70. PMID: 18320579
4. Differences in Galpha12- and Galpha13-mediated plasma membrane recruitment of p115-RhoGEF.  |  Bhattacharyya, R., et al. 2009. Cell Signal. 21: 996-1006. PMID: 19249348
5. Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF.  |  Chen, Z., et al. 2011. Protein Sci. 20: 107-17. PMID: 21064165
6. Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain.  |  Chen, Z., et al. 2012. J Biol Chem. 287: 25490-500. PMID: 22661716
7. Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF.  |  Scott, DW., et al. 2016. Mol Biol Cell. 27: 1420-30. PMID: 26985018
8. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation.  |  Singh, NK., et al. 2017. J Biol Chem. 292: 14080-14091. PMID: 28655771
9. Targeting Immunophenotypic Markers on Leukemic Stem Cells: How Lessons from Current Approaches and Advances in the Leukemia Stem Cell (LSC) Model Can Inform Better Strategies for Treating Acute Myeloid Leukemia (AML).  |  Mitchell, K. and Steidl, U. 2020. Cold Spring Harb Perspect Med. 10: PMID: 31451539
10. RAB27B-regulated exosomes mediate LSC maintenance via resistance to senescence and crosstalk with the microenvironment.  |  Chen, Y., et al. 2024. Leukemia. 38: 266-280. PMID: 38036630

The preferred RhoGEF p115 antibody is RhoGEF p115 Antibody (C-9): sc-74565.