rhodopsin Antibody (RET-P1) recommended for detection of rhodopsin of mouse, rat and human origin by WB, IP, IF and IHC(P)

rhodopsin Antibody (RET-P1): sc-57433

rhodopsin Antibody (RET-P1) is rated 4.7 out of 5 by 3.
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Datasheets

    • rhodopsin Antibody (RET-P1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a membrane preparation from adult retina of rat origin
    • recommended for detection of rhodopsin of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See rhodopsin (1D4): sc-57432 for rhodopsin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using rhodopsin (RET-P1): sc-57433 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from band is clean and specific This antibody is good quality for WB analysis, I got clean band 1:400 recommend.
Date published: 2017-06-11
Rated 5 out of 5 by from Great antibody for IHC(P) The rhodopsin Antibody (RET-P1): sc-57433 provides excellent data for IHC with formalin fixed, paraffin-embedded tissue sections showing cytoplasmic and membrane localized staining.
Date published: 2017-01-16
Rated 4 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of rhodopsin expression in rat eye and mouse eye tissue extracts. -SCBT QC
Date published: 2014-07-21
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