Anti-rhodopsin Antibody (1D4) is a mouse monoclonal IgG1 (kappa light chain) rhodopsin antibody provided at 200 µg/ml
raised against full length rhodopsin of bovine origin
Anti-rhodopsin Antibody (1D4) is recommended for detection of rhodopsin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
Anti-rhodopsin Antibody (1D4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of rhodopsin (1D4): sc-57432.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
rhodopsin Antibody (1D4) is a high quality monoclonal rhodopsin antibody (also designated rhodopsin antibody) suitable for the detection of the rhodopsin protein of mouse, rat and human origin. rhodopsin Antibody (1D4) is available as both the non-conjugated anti-rhodopsin antibody form, as well as multiple conjugated forms of anti-rhodopsin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. G protein-coupled receptors (GPCRs), which are characterized as containing seven transmembrane α helices, elicit G protein-mediated signaling cascades in response to a variety of stimuli. The opsin subfamily, which represents approximately 90 percent of all GPCRs, is comprised of photoreceptors that are activated by light, which include the red-, green- and blue-sensitive opsins and rhodopsin. The opsin subfamily consists of an apoprotein covalently linked to 11-cis-retinal, which undergoes isomerization upon the absorption of photons. This isomerization leads to a conformational change of the protein which results in the activation of hundreds of G proteins. Specifically, rhodopsin exhibits a maximal absorption at 495 nm and mediates vision in dim light. Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Would this antibody recognize the 1D4 tag in fusion protein in western blot?
Asked by: rogomca
Thank you for your question. This is a mouse monoclonal antibody that is recommended for detection of the protein rhodopsin. The clone name for this antibody is “1D4”, which is also known as the “1D4” epitope of rhodopsin. We have not tested this antibody for detection of any 1D4 fusion proteins.
Answered by: Technical Support
Date published: 2019-08-17
Can this mouse monoclonal antibody be used in IF or IHC on mouse tissue without background or non-specific staining issues?
Asked by: Anonymous
Thank you for your question. Yes, it can be used successfully for this application. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody rhodopsin (1D4): sc-57432 is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Rated 4 out of
Visualizing Endogenous Rhodopsin in RPE CellsUseful antibody for IF labeling of endogenous rhodopsin in RPE cells. Cells permeabilized for 30min in PBStween then blocked in 5%BSA. Antibody used at 1:200 overnight at 4 degrees.
Date published: 2020-02-14
Rated 4 out of
Antibody for IFI used this antibody on OCT embedded frozen retinal sections (mouse). Blocked for 1 hour with 10% goat serum buffer. Incubated with primary antibody overnight at the a concentration of 1:100. Incubated with secondary antibody (alexa fluor 568 anti-mouse) at a concentration of 1:1000. Staining was fairly robust but not completely consistent between all samples.
Date published: 2018-03-21
Rated 2 out of
Poor specificity in normal canine WBAntibody was used for WB with normal canine tissue. Very low specificity.
Date published: 2017-08-18
Rated 5 out of
Great reactivity.This antibody will give you strong and specific signals in your blotting and staining. It even cross-reacts with additional species. It has been published with over 15 times.
Date published: 2017-01-11
Rated 5 out of
Outstanding!!Great product for both western blot and ihc cell staining applications
Date published: 2017-01-05
Rated 4 out of
DecentI bought this antibody for WB of rhodopsin in Y79 cells. So far it works as advertised.
Date published: 2016-12-12
Rated 4 out of
Good specificity and signal strength withGood specificity and signal strength with some background in mouse eye and rat eye tissue extracts. -SCBT QC
Date published: 2015-06-01
Rated 5 out of
This antibody was used in a publication forThis antibody was used in a publication for WB on human HEK 293 and ARPE-19 cells - SCBT Publication Review
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