rhodopsin Antibody (1D4) recommended for detection of rhodopsin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

rhodopsin Antibody (1D4): sc-57432

rhodopsin Antibody (1D4) is rated 4.4 out of 5 by 11.
  • y_2020, m_3, d_29, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_en_US, sid_57432, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 127ms
  • REVIEWS, PRODUCT
5
6
4
4
3
0
2
1
1
0

Datasheets

    • Anti-rhodopsin Antibody (1D4) is a mouse monoclonal IgG1 (kappa light chain) rhodopsin antibody provided at 200 µg/ml
    • raised against full length rhodopsin of bovine origin
    • Anti-rhodopsin Antibody (1D4) is recommended for detection of rhodopsin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
    • Anti-rhodopsin Antibody (1D4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of rhodopsin (1D4): sc-57432.
Submit a review for this product and receive 15 CruzCredits

Would this antibody recognize the 1D4 tag in fusion protein in western blot?

Asked by: rogomca
Thank you for your question. This is a mouse monoclonal antibody that is recommended for detection of the protein rhodopsin. The clone name for this antibody is “1D4”, which is also known as the “1D4” epitope of rhodopsin. We have not tested this antibody for detection of any 1D4 fusion proteins.
Answered by: Technical Support
Date published: 2019-08-17

Can this mouse monoclonal antibody be used in IF or IHC on mouse tissue without background or non-specific staining issues?

Asked by: Anonymous
Thank you for your question. Yes, it can be used successfully for this application. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody rhodopsin (1D4): sc-57432 is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 10
Date published: 2017-01-09
  • y_2020, m_3, d_29, h_14CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_57432, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 143ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Visualizing Endogenous Rhodopsin in RPE Cells Useful antibody for IF labeling of endogenous rhodopsin in RPE cells. Cells permeabilized for 30min in PBStween then blocked in 5%BSA. Antibody used at 1:200 overnight at 4 degrees.
Date published: 2020-02-14
Rated 4 out of 5 by from Antibody for IF I used this antibody on OCT embedded frozen retinal sections (mouse). Blocked for 1 hour with 10% goat serum buffer. Incubated with primary antibody overnight at the a concentration of 1:100. Incubated with secondary antibody (alexa fluor 568 anti-mouse) at a concentration of 1:1000. Staining was fairly robust but not completely consistent between all samples.
Date published: 2018-03-21
Rated 2 out of 5 by from Poor specificity in normal canine WB Antibody was used for WB with normal canine tissue. Very low specificity.
Date published: 2017-08-18
Rated 5 out of 5 by from Great reactivity. This antibody will give you strong and specific signals in your blotting and staining. It even cross-reacts with additional species. It has been published with over 15 times.
Date published: 2017-01-11
Rated 5 out of 5 by from Outstanding!! Great product for both western blot and ihc cell staining applications
Date published: 2017-01-05
Rated 4 out of 5 by from Decent I bought this antibody for WB of rhodopsin in Y79 cells. So far it works as advertised.
Date published: 2016-12-12
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background in mouse eye and rat eye tissue extracts. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for WB on human HEK 293 and ARPE-19 cells - SCBT Publication Review
Date published: 2015-04-04
  • y_2020, m_3, d_29, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_en_US, sid_57432, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.