Rhodanese Antibody (G-7): sc-271883
- Rhodanese Antibody (G-7) is a mouse monoclonal IgG1 κ Rhodanese antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 37-65 near the N-terminus of Rhodanese of human origin
- Rhodanese Antibody (G-7) is recommended for detection of Rhodanese of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- Anti-Rhodanese Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Rhodanese Antibody (G-7) for WB applications. This reagent is now offered in a bundle with Rhodanese Antibody (G-7) (see ordering information below).
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Rhodanese Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rhodanese protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rhodanese (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rhodanese, also known as thiosulfate sulfurtransferase, is a crucial mitochondrial matrix enzyme encoded by nuclear DNA, consisting of 297 amino acids. Rhodanese (G-7) monoclonal antibody recognizes an enzyme that plays a vital role in detoxifying cyanide by converting it into less harmful thiocyanate, which is essential for cellular protection against cyanide toxicity. Additionally, Rhodanese is involved in the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes, highlighting its importance in various metabolic processes. First identified in human red blood cells in 1956 and later crystallized from beef liver, Rhodanese activity is particularly significant in mammals, where this enzyme facilitates cyanide detoxification. A deficiency in Rhodanese activity has been linked to Leber′s optic neuropathy, a hereditary condition that can lead to vision loss, emphasizing this enzyme′s critical role in human health. The gene encoding Rhodanese is located on human chromosome 22q11.2-qter, and red cell and tissue forms of Rhodanese are determined by separate genes, suggesting a complex regulation of expression across different tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rhodanese Antibody (G-7) References:
- Decreased expression of genes involved in sulfur amino acid metabolism in frataxin-deficient cells. | Tan, G., et al. 2003. Hum Mol Genet. 12: 1699-711. PMID: 12837693
- Different contributions of the three CXXC motifs of human protein-disulfide isomerase-related protein to isomerase activity and oxidative refolding. | Horibe, T., et al. 2004. J Biol Chem. 279: 4604-11. PMID: 14627699
- Some properties of human small heat shock protein Hsp22 (H11 or HspB8). | Kim, MV., et al. 2004. Biochem Biophys Res Commun. 315: 796-801. PMID: 14985082
- Evidence for the physiological role of a rhodanese-like protein for the biosynthesis of the molybdenum cofactor in humans. | Matthies, A., et al. 2004. Proc Natl Acad Sci U S A. 101: 5946-51. PMID: 15073332
- Synthesis of rhodanese in Hep 3B cells. | Pallini, R., et al. 1990. Mol Cell Biochem. 93: 61-7. PMID: 1691817
- Cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes. | Pallini, R., et al. 1991. Biochem Biophys Res Commun. 180: 887-93. PMID: 1953758
- Thiosulphate-sulphur transferase (rhodanese) deficiency in Leber's hereditary optic atrophy. | Cagianut, B., et al. 1981. Lancet. 2: 981-2. PMID: 6117741
- Cloning and expression of human liver rhodanese cDNA. | Aita, N., et al. 1997. Biochem Biophys Res Commun. 231: 56-60. PMID: 9070219
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rhodanese Antibody (G-7) | sc-271883 | 200 µg/ml | $322.00 | |||
Rhodanese Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-525834 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rhodanese Antibody (G-7) AC | sc-271883 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rhodanese Antibody (G-7) HRP | sc-271883 HRP | 200 µg/ml | $322.00 | |||
Rhodanese Antibody (G-7) FITC | sc-271883 FITC | 200 µg/ml | $336.00 | |||
Rhodanese Antibody (G-7) PE | sc-271883 PE | 200 µg/ml | $349.00 | |||
Rhodanese Antibody (G-7) Alexa Fluor® 488 | sc-271883 AF488 | 200 µg/ml | $364.00 | |||
Rhodanese Antibody (G-7) Alexa Fluor® 546 | sc-271883 AF546 | 200 µg/ml | $364.00 | |||
Rhodanese Antibody (G-7) Alexa Fluor® 594 | sc-271883 AF594 | 200 µg/ml | $364.00 | |||
Rhodanese Antibody (G-7) Alexa Fluor® 647 | sc-271883 AF647 | 200 µg/ml | $364.00 | |||
Rhodanese Antibody (G-7) Alexa Fluor® 680 | sc-271883 AF680 | 200 µg/ml | $364.00 | |||
Rhodanese Antibody (G-7) Alexa Fluor® 790 | sc-271883 AF790 | 200 µg/ml | $364.00 | |||
Rhodanese (G-7) Neutralizing Peptide | sc-271883 P | 100 µg/0.5 ml | $69.00 |