RHOBTB3 CRISPR/Cas9 KO Plasmid (h): sc-406242
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RHOBTB3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RHOBTB3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RHOBTB3 CRISPR/Cas9 KO Plasmid (h) | sc-406242 | 20 µg | $397.00 |
Overview
RHOBTB3 encodes an atypical Rho family GTPase with BTB domains that functions as an adaptor linking membrane trafficking to ubiquitin-dependent protein turnover. RHOBTB3 has been implicated in Golgi and endosomal organization, vesicle transport, and regulation of cell-cycle progression through interactions with Cullin3-based E3 ubiquitin ligase complexes. By influencing intracellular trafficking and proteostasis, RHOBTB3 can modulate signaling outputs that depend on receptor recycling and compartmentalized small GTPase activity. Dysregulation of these processes has been associated with altered proliferation and migration phenotypes reported in cancer-related studies, supporting its relevance for mechanistic oncology and cell biology research.
RHOBTB3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RHOBTB3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RHOBTB3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RHOBTB3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RHOBTB3 protein expression.
This CRISPR knockout system enables efficient generation of RHOBTB3-deficient cell models for investigation of RHOBTB3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RHOBTB3 exon(s) critical for RHOBTB3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RHOBTB3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RHOBTB3 CRISPR/Cas9 KO Plasmid (h) and RHOBTB3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RHOBTB3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RHOBTB3 HDR Plasmid (h) and RHOBTB3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RHOBTB3 homology arms to support homology-directed repair at defined RHOBTB3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.