Rho GDIα Antibody (G-2): sc-373724

Rho GDIα Antibody (G-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Rho GDI alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Rho GDIα antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rho GDIα, also known as ARHGDIA or GDIA1, is a 204 amino acid protein that plays a crucial role in regulating the activity of Rho proteins, which are essential for various cellular processes such as proliferation, cytoskeletal dynamics, and secretion. Located primarily in the cytoplasm, Rho GDIα functions by inhibiting the dissociation of guanosine diphosphate (GDP) from Rho proteins, thereby preventing the activation of these proteins by guanosine triphosphate (GTP). This regulatory mechanism is vital for maintaining cellular homeostasis and ensuring proper signaling pathways are activated at the right times. Notably, Rho GDIα can undergo post-translational modification through phosphorylation at Ser 101 by p21-activated kinase (αPAK), which inhibits its activity and may lead to positive feedback regulation of certain target proteins. This intricate balance of activation and inhibition underscores the importance of Rho GDIα in cellular signaling and its potential implications in various diseases, making anti-Rho GDIα antibody (G-2) an invaluable tool for researchers studying these pathways.

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Rho GDIα Antibody (G-2) References:

1. Progressive impairment of kidneys and reproductive organs in mice lacking Rho GDIalpha.  |  Togawa, A., et al. 1999. Oncogene. 18: 5373-80. PMID: 10498891
2. Mechanism of NADPH oxidase activation by the Rac/Rho-GDI complex.  |  Di-Poï, N., et al. 2001. Biochemistry. 40: 10014-22. PMID: 11513579
3. Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase.  |  DerMardirossian, C., et al. 2004. Mol Cell. 15: 117-27. PMID: 15225553
4. Uncoupling of inhibitory and shuttling functions of rho GDP dissociation inhibitors.  |  Dransart, E., et al. 2005. J Biol Chem. 280: 4674-83. PMID: 15513926
5. Phosphorylation of RhoGDI by Src regulates Rho GTPase binding and cytosol-membrane cycling.  |  DerMardirossian, C., et al. 2006. Mol Biol Cell. 17: 4760-8. PMID: 16943322
6. Rho GDP dissociation inhibitor alpha interacts with estrogen receptor alpha and influences estrogen responsiveness.  |  El Marzouk, S., et al. 2007. J Mol Endocrinol. 39: 249-59. PMID: 17909265
7. Activity of the Bcr GTPase-activating domain is regulated through direct protein/protein interaction with the Rho guanine nucleotide dissociation inhibitor.  |  Kweon, SM., et al. 2008. J Biol Chem. 283: 3023-3030. PMID: 18070886
8. Regulation of RhoA signaling by the cAMP-dependent phosphorylation of RhoGDIα.  |  Oishi, A., et al. 2012. J Biol Chem. 287: 38705-15. PMID: 23012358
9. Structural and Mechanistic Insights into the Regulation of the Fundamental Rho Regulator RhoGDIα by Lysine Acetylation.  |  Kuhlmann, N., et al. 2016. J Biol Chem. 291: 5484-5499. PMID: 26719334
10. RhoGDIα suppresses self-renewal and tumorigenesis of glioma stem cells.  |  Wu, F., et al. 2016. Oncotarget. 7: 61619-61629. PMID: 27557508
11. Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton.  |  Leffers, H., et al. 1993. Exp Cell Res. 209: 165-74. PMID: 8262133
12. A somatic cell hybrid panel for distal 17q: GDIA1 maps to 17q25.3.  |  Wagner, T., et al. 1997. Cytogenet Cell Genet. 76: 172-5. PMID: 9186513

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rho GDIα Antibody (G-2) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-Rho GDIα Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rho GDIα Antibody (G-2) Specifications

Alternate Product Name: Rho GDIα

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2-29

Epitope Target: Rho GDIα

Epitope Species: human

Product Reactivity/Detection: Rho GDIα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Rho GDIα Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Ly-GDI/RhoGDI2/ARHGDIB antibody is Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12): sc-376473.