Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RhoB CRISPR/Cas9 KO Plasmid (h): sc-400875
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RhoB CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RhoB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RhoB Antibody (C-5): sc-8048
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RhoB CRISPR/Cas9 KO Plasmid (h) | sc-400875 | 20 µg | $397.00 |
Overview
RHOB encodes RhoB, a prenylated small GTPase in the Rho family that regulates actin cytoskeleton dynamics, endocytic trafficking, and membrane receptor turnover. RhoB cycles between GDP- and GTP-bound states to modulate signaling nodes linked to cell adhesion, migration, vesicle transport, and stress responses, with functional connections to pathways including Rho/ROCK and broader MAPK- and PI3K-influenced processes. Unlike closely related Rho GTPases, RhoB is enriched on endosomal membranes and is frequently induced by cellular stress, positioning it as a key mediator of signal compartmentalization. Altered RHOB expression or activity has been associated with cancer-related phenotypes, inflammatory signaling, and vascular biology, making it relevant for mechanistic studies of proliferation, invasion, and receptor-driven signaling.
RhoB CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RHOB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RHOB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RHOB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RhoB protein expression.
This CRISPR knockout system enables efficient generation of RHOB-deficient cell models for investigation of RhoB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RHOB exon(s) critical for RhoB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RHOB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RhoB CRISPR/Cas9 KO Plasmid (h) and RhoB CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RHOB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RhoB HDR Plasmid (h) and RhoB HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RHOB homology arms to support homology-directed repair at defined RHOB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.