RHBDL6 CRISPR/Cas9 KO Plasmid (h): sc-404212
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RHBDL6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RHBDL6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RHBDL6 CRISPR/Cas9 KO Plasmid (h) | sc-404212 | 20 µg | $397.00 |
Overview
RHBDF2 encodes RHBDL6, an intramembrane serine protease of the rhomboid family that localizes to endomembranes and contributes to regulated proteolysis of membrane-associated substrates. RHBDL6 activity has been linked to protein quality control, trafficking, and turnover of signaling components, integrating with ER-associated degradation and stress-adaptive programs that shape cellular proteostasis. Through these mechanisms, RHBDL6 can influence growth factor and inflammatory signaling outputs, including pathways connected to EGFR ligand availability and innate immune modulation. Dysregulation of rhomboid protease–mediated processing has been associated with pathological remodeling of signaling networks in cancer biology and inflammatory disease models, supporting investigation of RHBDL6-dependent proteolysis in disease-relevant contexts.
RHBDL6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RHBDF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RHBDF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RHBDF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RHBDL6 protein expression.
This CRISPR knockout system enables efficient generation of RHBDF2-deficient cell models for investigation of RHBDL6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RHBDF2 exon(s) critical for RHBDL6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RHBDF2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RHBDL6 CRISPR/Cas9 KO Plasmid (h) and RHBDL6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RHBDF2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RHBDL6 HDR Plasmid (h) and RHBDL6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RHBDF2 homology arms to support homology-directed repair at defined RHBDF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.