Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RGS6 CRISPR/Cas9 KO Plasmid (h2): sc-404102-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RGS6 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RGS6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGS6 CRISPR/Cas9 KO Plasmid (h2) | sc-404102-KO-2 | 20 µg | $397.00 |
Overview
RGS6 (regulator of G protein signaling 6) is a human RGS family protein that accelerates GTP hydrolysis on activated Gα subunits, thereby terminating GPCR-driven signaling. It modulates second-messenger pathways including cAMP/PKA and calcium-dependent signaling, shaping neuronal excitability, cardiac electrophysiology, and responses to neurotransmitters. RGS6 has been linked to regulation of apoptosis and oxidative stress responses, and its dysregulation is studied in the context of neurodegenerative processes, arrhythmia-related signaling, and tumor-associated pathways. As a GPCR signal terminator, RGS6 is a useful node for dissecting pathway dynamics downstream of receptor activation and for mapping crosstalk with MAPK and stress-response networks.
RGS6 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the RGS6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RGS6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RGS6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RGS6 protein expression.
This CRISPR knockout system enables efficient generation of RGS6-deficient cell models for investigation of RGS6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RGS6 exon(s) critical for RGS6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RGS6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RGS6 CRISPR/Cas9 KO Plasmid (h) and RGS6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RGS6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RGS6 HDR Plasmid (h) and RGS6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RGS6 homology arms to support homology-directed repair at defined RGS6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.