RGPD5 CRISPR/Cas9 KO Plasmid (h): sc-401666
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RGPD5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RGPD5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGPD5 CRISPR/Cas9 KO Plasmid (h) | sc-401666 | 20 µg | $397.00 |
Overview
RGPD5 (RANBP2-like and GRIP domain containing 5) is a primate-specific member of the RGPD gene family implicated in nucleocytoplasmic transport processes through homology to RANBP2/NUP358-associated domains. Proteins in this family are thought to influence Ran GTPase–regulated trafficking, mitotic progression, and spatial organization of protein complexes at the nuclear envelope, thereby impacting global gene expression programs. RGPD5 is frequently discussed in the context of segmental duplications on chromosome 2 and copy-number variability, features that complicate locus-specific interpretation in genomic studies. Dysregulation or structural variation in RGPD family regions has been explored in association with proliferative phenotypes and genome instability in cancer genomics datasets, supporting its use as a target for mechanistic studies of nuclear transport and cell-cycle control.
RGPD5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RGPD5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RGPD5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RGPD5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RGPD5 protein expression.
This CRISPR knockout system enables efficient generation of RGPD5-deficient cell models for investigation of RGPD5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RGPD5 exon(s) critical for RGPD5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RGPD5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RGPD5 CRISPR/Cas9 KO Plasmid (h) and RGPD5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RGPD5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RGPD5 HDR Plasmid (h) and RGPD5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RGPD5 homology arms to support homology-directed repair at defined RGPD5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.