Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RG9MTD1 Double Nickase Plasmid (h): sc-418590-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RG9MTD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RG9MTD1 Double Nickase Plasmid (h) and RG9MTD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TRMT10C. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RG9MTD1 Antibody (A-8): sc-515289
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RG9MTD1 Double Nickase Plasmid (h) | sc-418590-NIC | 20 µg | $410.00 |
Human TRMT10C encodes the mitochondrial tRNA:m1G9 methyltransferase catalytic subunit, while RG9MTD1 (MRPP1) functions with MRPP2 (HSD17B10) and MRPP3 (KIAA0391) in the mitochondrial RNase P complex that couples tRNA processing with m1G9 methylation. This activity is integral to mitochondrial RNA maturation, mitoribosome function, and efficient oxidative phosphorylation by supporting accurate mitochondrial translation. Disruption of TRMT10C/RG9MTD1-dependent tRNA processing perturbs respiratory chain biogenesis and cellular energy homeostasis, linking the pathway to mitochondrial dysfunction phenotypes observed in neurometabolic disease contexts. As a result, TRMT10C/RG9MTD1 is frequently studied in models of mitochondrial gene expression, proteostasis, and stress responses that converge on electron transport and apoptosis-related signaling.
RG9MTD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TRMT10C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TRMT10C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TRMT10C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TRMT10C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.