RFX5 Antibody (C-3): sc-271756

RFX5 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RFX5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-RFX5 antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RFX5 protein plays a crucial role in the regulation of major histocompatibility complex (MHC) class II gene expression, which is vital for the immune response as RFX5 presents antigens to T cells, thereby initiating adaptive immunity. RFX5 is part of a larger protein complex that includes RFX1, RFX2, and RFX3, which bind to specific DNA sequences in the promoters of MHC class II genes, facilitating their transcription in response to signals such as interferon-γ. Proper functioning of RFX5 and associated proteins is essential for the immune system′s ability to recognize and respond to pathogens, making RFX5 a significant target for research in immunology and potential therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RFX5 Antibody (C-3) References:

1. Orientation and positional mapping of the subunits of the multicomponent transcription factors RFX and X2BP to the major histocompatibility complex class II transcriptional enhancer.  |  Westerheide, SD. and Boss, JM. 1999. Nucleic Acids Res. 27: 1635-41. PMID: 10075994
2. Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding.  |  Gajiwala, KS., et al. 2000. Nature. 403: 916-21. PMID: 10706293
3. Novel mutations within the RFX-B gene and partial rescue of MHC and related genes through exogenous class II transactivator in RFX-B-deficient cells.  |  Nagarajan, UM., et al. 2000. J Immunol. 164: 3666-74. PMID: 10725724
4. Functional organization of the hepatitis B virus enhancer.  |  Dikstein, R., et al. 1990. Mol Cell Biol. 10: 3683-9. PMID: 2355919
5. Assembly of functional regulatory complexes on MHC class II promoters in vivo.  |  Fontes, JD., et al. 1997. J Mol Biol. 270: 336-45. PMID: 9237901
6. The transcriptional activation and repression domains of RFX1, a context-dependent regulator, can mutually neutralize their activities.  |  Katan, Y., et al. 1997. Nucleic Acids Res. 25: 3621-8. PMID: 9278482
7. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes.  |  Katan-Khaykovich, Y. and Shaul, Y. 1998. J Biol Chem. 273: 24504-12. PMID: 9733744
8. A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients.  |  Masternak, K., et al. 1998. Nat Genet. 20: 273-7. PMID: 9806546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RFX5 Antibody (C-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-RFX5 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RFX5 Antibody (C-3) Specifications

Alternate Product Name: RFX5

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 161-460

Epitope Target: RFX5

Epitope Species: human

Product Reactivity/Detection: RFX5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for RFX5 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent