Date published: 2026-3-6

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RFX5 Antibody (A-10): sc-271757

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Datasheets
  • RFX5 Antibody (A-10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 161-460 mapping within an internal region of RFX5 of human origin
  • recommended for detection of RFX5 of human origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-271757 X, 200 µg/0.1 ml)
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RFX5 Antibody (A-10). This work is in progress.
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    RFX5 Antibody (A-10) is a mouse monoclonal IgG1 antibody that detects RFX5 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RFX5 is a crucial component of the regulatory factor X (RFX) complex, which plays a significant role in the transcriptional regulation of major histocompatibility complex (MHC) class II genes. RFX5 is primarily located in the nucleus, where RFX5 binds to specific DNA sequences in the promoters of MHC class II genes, facilitating their expression in response to immune signals, particularly interferon-γ. Proper functioning of RFX5 is vital for the immune system, as RFX5 ensures the presentation of antigens to T cells, thereby influencing adaptive immune responses. Dysregulation of RFX5 can lead to impaired immune function and has been associated with various autoimmune diseases. Anti-RFX5 antibody (A-10) provides reliable detection and analysis of RFX5, enabling researchers to explore RFX5′s role in immune regulation and potential implications in disease.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    RFX5 Antibody (A-10)

    sc-271757
    200 µg/ml
    $322.00

    RFX5 Antibody (A-10) X

    sc-271757 X
    200 µg/0.1 ml
    $322.00

    What is the recommended starting dilution for Western blot for RFX5 (A-10): sc-271757 monoclonal antibody?

    Asked by: Trav11
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-271757.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Great Western blot data of RFX5 expression in nonGreat Western blot data of RFX5 expression in non-transfected and human RFX5 transfected 293 whole cell lysates and Ramos and Jurkat nuclear extracts. -SCBT QC
    Date published: 2014-12-08
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    RFX5 Antibody (A-10) is rated 5.0 out of 5 by 1.
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