RFX1 Antibody (D-3): sc-514849

RFX1 Antibody (D-3) is a mouse monoclonal IgG1 antibody that detects RFX1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RFX1 is a crucial transcription factor that plays a significant role in the regulation of major histocompatibility complex (MHC) class II genes, which are essential for the immune response. RFX1 is primarily located in the nucleus, where RFX1 forms a complex with other RFX proteins, such as RFX2 and RFX3, to bind to specific DNA sequences in the promoters of target genes. This nuclear localization enables RFX1 to directly influence gene expression in response to various stimuli, including interferon-gamma, thereby modulating the immune system′s ability to present antigens and activate T cells. The interaction of RFX1 with other proteins in the RFX complex enhances RFX1′s ability to regulate gene transcription, making RFX1 a key player in immune system function and a potential target for therapeutic interventions in immune-related diseases. Anti-RFX1 antibody (D-3) is an invaluable tool for researchers studying the mechanisms of immune regulation and the role of transcription factors in cellular responses.

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RFX1 Antibody (D-3) References:

1. Activation of protein kinase C induces nuclear translocation of RFX1 and down-regulates c-myc via an intron 1 X box in undifferentiated leukemia HL-60 cells.  |  Chen, L., et al. 2000. J Biol Chem. 275: 32227-33. PMID: 10918054
2. RFX1 and NF-1 associate with P sequences of the human growth hormone locus in pituitary chromatin.  |  Norquay, LD., et al. 2003. Mol Endocrinol. 17: 1027-38. PMID: 12624117
3. Identification of an epigenetically silenced gene, RFX1, in human glioma cells using restriction landmark genomic scanning.  |  Ohashi, Y., et al. 2004. Oncogene. 23: 7772-9. PMID: 15334059
4. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells.  |  Su, JC., et al. 2014. Oncotarget. 5: 4909-19. PMID: 24952874
5. Recruitment of the protein phosphatase-1 catalytic subunit to promoters by the dual-function transcription factor RFX1.  |  Lubelsky, Y. and Shaul, Y. 2019. Biochem Biophys Res Commun. 509: 1015-1020. PMID: 30654936
6. The myc intron-binding polypeptide associates with RFX1 in vivo and binds to the major histocompatibility complex class II promoter region, to the hepatitis B virus enhancer, and to regulatory regions of several distinct viral genes.  |  Reinhold, W., et al. 1995. Mol Cell Biol. 15: 3041-8. PMID: 7760800
7. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins.  |  Reith, W., et al. 1994. Mol Cell Biol. 14: 1230-44. PMID: 8289803
8. RFX1 is identical to enhancer factor C and functions as a transactivator of the hepatitis B virus enhancer.  |  Siegrist, CA., et al. 1993. Mol Cell Biol. 13: 6375-84. PMID: 8413236
9. The transcriptional activation and repression domains of RFX1, a context-dependent regulator, can mutually neutralize their activities.  |  Katan, Y., et al. 1997. Nucleic Acids Res. 25: 3621-8. PMID: 9278482
10. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes.  |  Katan-Khaykovich, Y. and Shaul, Y. 1998. J Biol Chem. 273: 24504-12. PMID: 9733744

The preferred RFX1 antibody is RFX1 Antibody (F-6): sc-374270.