REV1 CRISPR/Cas9 KO Plasmid (m): sc-425035
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- REV1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the REV1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REV1 CRISPR/Cas9 KO Plasmid (m) | sc-425035 | 20 µg | $397.00 |
Overview
Rev1 encodes REV1, a Y-family DNA polymerase that functions as a specialized scaffold and deoxycytidyl transferase in translesion DNA synthesis, enabling replication to proceed across bulky DNA lesions. REV1 coordinates lesion bypass by interacting with other TLS polymerases and integrates with DNA damage response networks that preserve fork progression and genome integrity. In mouse cells, Rev1 activity influences mutagenesis rates and the balance between error-prone and error-free lesion processing during replication stress. Dysregulated REV1-dependent pathways are relevant to studies of genome instability, carcinogenesis mechanisms, and cellular sensitivity to genotoxic stressors.
REV1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rev1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rev1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rev1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish REV1 protein expression.
This CRISPR knockout system enables efficient generation of Rev1-deficient cell models for investigation of REV1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rev1 exon(s) critical for REV1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rev1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by REV1 CRISPR/Cas9 KO Plasmid (m) and REV1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rev1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by REV1 HDR Plasmid (m) and REV1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rev1 homology arms to support homology-directed repair at defined Rev1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.