REV1 Antibody (A-11): sc-393022

REV1 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects REV1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). REV1 Antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. REV1 plays a crucial role in DNA repair and replication, specifically in translesion synthesis, which allows DNA polymerases to bypass lesions that would otherwise stall replication. This function is vital for maintaining genomic stability, especially in response to DNA damage caused by environmental factors or cellular processes. The human REV1 gene, located between chromosomal loci 2q11.1 and 2q11.2, is expressed in a wide range of tissues, highlighting REV1′s importance in cellular responses to DNA damage across different biological contexts. As a deoxycytidyl transferase, REV1 facilitates the insertion of deoxycytidine monophosphate opposite damaged DNA bases, enabling the continuation of DNA synthesis despite the presence of lesions. This mechanism is particularly significant in the context of mutagenesis, as REV1 allows cells to tolerate and adapt to DNA damage, which can have implications for cancer development and treatment resistance.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

REV1 Antibody (A-11) References:

1. The human REV1 gene codes for a DNA template-dependent dCMP transferase.  |  Lin, W., et al. 1999. Nucleic Acids Res. 27: 4468-75. PMID: 10536157
2. Distinct roles for Rev1p and Rev7p during translesion synthesis in Saccharomyces cerevisiae.  |  Baynton, K., et al. 1999. Mol Microbiol. 34: 124-33. PMID: 10540291
3. The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light.  |  Gibbs, PE., et al. 2000. Proc Natl Acad Sci U S A. 97: 4186-91. PMID: 10760286
4. Evidence for a second function for Saccharomyces cerevisiae Rev1p.  |  Nelson, JR., et al. 2000. Mol Microbiol. 37: 549-54. PMID: 10931348
5. Mutagenesis in eukaryotes dependent on DNA polymerase zeta and Rev1p.  |  Lawrence, CW. and Maher, VM. 2001. Philos Trans R Soc Lond B Biol Sci. 356: 41-6. PMID: 11205328
6. Human Rev1 polymerase disrupts G-quadruplex DNA.  |  Eddy, S., et al. 2014. Nucleic Acids Res. 42: 3272-85. PMID: 24366879
7. REV1-Polζ maintains the viability of homologous recombination-deficient cancer cells through mutagenic repair of PRIMPOL-dependent ssDNA gaps.  |  Taglialatela, A., et al. 2021. Mol Cell. 81: 4008-4025.e7. PMID: 34508659
8. REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.  |  Chen, Y., et al. 2022. Cell Death Dis. 13: 110. PMID: 35115490
9. The Catalytic Activity of Human REV1 on Undamaged and Damaged DNA.  |  Stolyarenko, AD., et al. 2024. Int J Mol Sci. 25: PMID: 38612916
10. USP9X-mediated REV1 deubiquitination promotes lung cancer radioresistance via the action of REV1 as a Rad18 molecular scaffold for cystathionine γ-lyase.  |  Chen, Y., et al. 2024. J Biomed Sci. 31: 55. PMID: 38802791
11. Loss-of-function mutation of REV1 (p.R704Q) mediates cetuximab primary resistance by activating autophagy in RAS-wild type metastatic colorectal cancer.  |  Zhu, N., et al. 2024. Cancer Lett. 598: 217103. PMID: 38969162
12. Deoxycytidyl transferase activity of yeast REV1 protein.  |  Nelson, JR., et al. 1996. Nature. 382: 729-31. PMID: 8751446

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-REV1 Antibody (A-11) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-REV1 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

REV1 Antibody (A-11) Specifications

Alternate Product Name: REV1

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-380

Epitope Target: REV1

Epitope Species: human

Product Reactivity/Detection: REV1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for REV1 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent