Rev-erbβ Antibody (QK-6): sc-100911

Rev-erbβ Antibody (QK-6) is a mouse monoclonal IgG1 antibody that detects Rev-erbβ in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rev-erbβ, also known as NR1D2, plays a crucial role in regulating circadian rhythms and metabolic processes by acting as a transcriptional repressor. Rev-erbβ is primarily located in the nucleus, where Rev-erbβ binds to specific DNA sequences, thereby influencing the expression of genes involved in the circadian clock and energy homeostasis. Rev-erbβ′s nuclear localization enables direct interaction with other nuclear proteins, such as the retinoic acid receptor-related orphan receptor alpha (RORα), to modulate transcriptional activity. This interaction is vital for maintaining the balance of metabolic functions and ensuring proper physiological responses to environmental cues. Anti-Rev-erbβ antibody (QK-6) is an essential tool for researchers studying the intricate mechanisms of circadian regulation and its implications in various metabolic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rev-erbβ Antibody (QK-6) References:

1. Rev-erbα and Rev-erbβ coordinately protect the circadian clock and normal metabolic function.  |  Bugge, A., et al. 2012. Genes Dev. 26: 657-67. PMID: 22474260
2. Structure of REV-ERBβ ligand-binding domain bound to a porphyrin antagonist.  |  Matta-Camacho, E., et al. 2014. J Biol Chem. 289: 20054-66. PMID: 24872411
3. Domain-Specific Monoclonal Antibodies Against Human Rev-erbβ.  |  Chen, F., et al. 2017. Appl Biochem Biotechnol. 182: 978-989. PMID: 27987190
4. REV-ERBβ is required to maintain normal wakefulness and the wake-inducing effect of dual REV-ERB agonist SR9009.  |  Amador, A., et al. 2018. Biochem Pharmacol. 150: 1-8. PMID: 29355503
5. Distinct roles for REV-ERBα and REV-ERBβ in oxidative capacity and mitochondrial biogenesis in skeletal muscle.  |  Amador, A., et al. 2018. PLoS One. 13: e0196787. PMID: 29723273
6. REV-ERBα and REV-ERBβ function as key factors regulating Mammalian Circadian Output.  |  Ikeda, R., et al. 2019. Sci Rep. 9: 10171. PMID: 31308426
7. Pharmacological modulation and genetic deletion of REV-ERBα and REV-ERBβ regulates dendritic cell development.  |  Amir, M., et al. 2020. Biochem Biophys Res Commun. 527: 1000-1007. PMID: 32439175
8. Structural basis for heme-dependent NCoR binding to the transcriptional repressor REV-ERBβ.  |  Mosure, SA., et al. 2021. Sci Adv. 7: PMID: 33571111
9. Molecular Dynamics of Cobalt Protoporphyrin Antagonism of the Cancer Suppressor REV-ERBβ.  |  Fakih, TM., et al. 2021. Molecules. 26: PMID: 34071361
10. Functional Characterization of Circadian Nuclear Receptors REV-ERBα and REV-ERBβ in Human Osteosarcoma Cell Cultures.  |  Cho, H., et al. 2024. Int J Mol Sci. 25: PMID: 38255844

The preferred Rev-erbβ antibody is Rev-erbβ Antibody (D-8): sc-398252.