Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rev-erbα Double Nickase Plasmid (h): sc-401211-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rev-erbα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rev-erbα Double Nickase Plasmid (h) and Rev-erbα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR1D1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rev-erbα Antibody (E-12): sc-393215
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rev-erbα Double Nickase Plasmid (h) | sc-401211-NIC | 20 µg | $410.00 | |||
Rev-erbα Double Nickase Plasmid (h2) | sc-401211-NIC-2 | 20 µg | $410.00 |
NR1D1 encodes the nuclear receptor Rev-erbα, a heme-binding transcriptional repressor that integrates circadian timing with cellular metabolism. Rev-erbα modulates core clock circuitry by repressing target genes involved in the BMAL1/ARNTL axis and coordinates lipid and glucose homeostasis through crosstalk with metabolic regulators and nuclear receptor signaling. Through regulation of inflammatory gene programs and mitochondrial and metabolic pathways, NR1D1 activity is widely studied in contexts linking circadian disruption to metabolic and immune dysregulation. Altered NR1D1/Rev-erbα function has been associated in research literature with metabolic syndrome-related phenotypes, neurobehavioral processes, and tumor biology, supporting its value in mechanistic studies.
Rev-erbα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR1D1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR1D1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR1D1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR1D1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.