RESP18 CRISPR/Cas9 KO Plasmid (m): sc-422652
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RESP18 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RESP18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RESP18 CRISPR/Cas9 KO Plasmid (m) | sc-422652 | 20 µg | $397.00 |
Overview
Resp18 encodes RESP18, a neuroendocrine secretory granule–associated protein enriched in pancreatic islet cells and other regulated secretory tissues. RESP18 is implicated in secretory granule biogenesis and cargo sorting, supporting stimulus-dependent hormone release and maintenance of granule content. Through its linkage to regulated exocytosis and endocrine cell homeostasis, Resp18 provides a molecular entry point for studying pathways that control insulin granule maturation and secretion dynamics. Altered granule organization or hormone secretion phenotypes in experimental models make Resp18 relevant to metabolic and endocrine dysfunction research.
RESP18 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Resp18 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Resp18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Resp18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RESP18 protein expression.
This CRISPR knockout system enables efficient generation of Resp18-deficient cell models for investigation of RESP18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Resp18 exon(s) critical for RESP18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Resp18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RESP18 CRISPR/Cas9 KO Plasmid (m) and RESP18 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Resp18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RESP18 HDR Plasmid (m) and RESP18 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Resp18 homology arms to support homology-directed repair at defined Resp18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.