Rent2 Double Nickase Plasmid (m): sc-435860-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rent2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rent2 Double Nickase Plasmid (m) and Rent2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Upf2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rent2 Antibody (G-10): sc-374230
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rent2 Double Nickase Plasmid (m) | sc-435860-NIC | 20 µg | $410.00 | |||
Rent2 Double Nickase Plasmid (m2) | sc-435860-NIC-2 | 20 µg | $410.00 |
Mouse Upf2 (Rent2) encodes an essential RNA surveillance factor required for nonsense-mediated mRNA decay (NMD), a pathway that detects and degrades transcripts containing premature termination codons to maintain proteome fidelity. RENT2 functions with core NMD components such as UPF1 and UPF3 and couples translation termination to mRNP remodeling, influencing mRNA stability, alternative splicing outcomes, and stress-responsive transcriptome regulation. Upf2 activity impacts neuronal development, immune homeostasis, and germline integrity, and dysregulated NMD has been linked to neurodevelopmental phenotypes, cancer-relevant transcript buffering, and altered responses to genotoxic or proteotoxic stress. Experimental perturbation of Upf2 is widely used to interrogate RNA quality control, translational surveillance, and gene expression robustness in mammalian cells.
Rent2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Upf2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Upf2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Upf2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Upf2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.