Renin Antibody (B-12): sc-133145

Renin Antibody (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Renin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Renin antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Renin plays a crucial role as a highly specific endopeptidase that catalyzes the conversion of angiotensinogen into Angiotensin I, which is subsequently transformed into Angiotensin II by angiotensin-converting enzyme I (ACE). This process is vital for the regulation of blood pressure and electrolyte balance, making Renin a key player in the renin-angiotensin-aldosterone system (RAAS). The release of Renin is stimulated by sympathetic nervous system activation, renal artery hypotension, and reduced sodium delivery to the distal tubules of the kidney, highlighting its importance in maintaining cardiovascular homeostasis. Additionally, Renin is synthesized and secreted by specialized smooth muscle cells in the juxtaglomerular apparatus of the kidney, although Renin expression in other tissues, such as the brain, suggests potential roles in additional physiological processes that are still being explored.

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Renin Antibody (B-12) References:

1. Homeostasis in mice with genetically decreased angiotensinogen is primarily by an increased number of renin-producing cells.  |  Kim, HS., et al. 1999. J Biol Chem. 274: 14210-7. PMID: 10318840
2. Renin and the kidney.  |  Chonko, AM., et al. 1975. Nephron. 15: 279-305. PMID: 1101089
3. Microvascular angiogenesis and the renin-angiotensin system.  |  Greene, AS. and Amaral, SL. 2002. Curr Hypertens Rep. 4: 56-62. PMID: 11790293
4. The renin angiotensin system and kidney development.  |  Matsusaka, T., et al. 2002. Annu Rev Physiol. 64: 551-61. PMID: 11826279
5. Renin gene expression: the switch and the fingers.  |  Morris, BJ., et al. 2001. Clin Exp Pharmacol Physiol. 28: 1044-7. PMID: 11903312
6. HADHB, HuR, and CP1 bind to the distal 3'-untranslated region of human renin mRNA and differentially modulate renin expression.  |  Adams, DJ., et al. 2003. J Biol Chem. 278: 44894-903. PMID: 12933794
7. Candidate cis-elements for human renin gene expression in the promoter region.  |  Konoshita, T., et al. 2004. J Cell Biochem. 93: 327-36. PMID: 15368359
8. Renin-Independent hypermineralocorticoidism.  |  Young, WF. and Hogan, MJ. 1994. Trends Endocrinol Metab. 5: 97-106. PMID: 18407194
9. Physiology of kidney renin.  |  Castrop, H., et al. 2010. Physiol Rev. 90: 607-73. PMID: 20393195
10. Characterization of human prorenin expressed in mammalian cells from cloned cDNA.  |  Fritz, LC., et al. 1986. Proc Natl Acad Sci U S A. 83: 4114-8. PMID: 3520565
11. Renin and renin blockade have no role in complement activity.  |  Zhang, Y., et al. 2024. Kidney Int. 105: 328-337. PMID: 38008161
12. Ren-1 and Ren-2 loci are expressed in mouse kidney.  |  Field, LJ. and Gross, KW. 1985. Proc Natl Acad Sci U S A. 82: 6196-200. PMID: 3898081

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Renin Antibody (B-12) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-Renin Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Renin Antibody (B-12) Specifications

Alternate Product Name: REN

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 116-220

Epitope Target: Renin

Epitope Species: human

Product Reactivity/Detection: precursor and mature Renin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for Renin Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin