Relaxin Receptor 1 CRISPR/Cas9 KO Plasmid (h): sc-417299

Overview

RXFP1 encodes relaxin receptor 1, a GPCR that binds relaxin-family peptides to regulate cAMP/PKA and MAPK signaling, nitric oxide production, and transcriptional programs that influence extracellular matrix remodeling. In fibroblasts, smooth muscle, and vascular cells, RXFP1 activity modulates cell migration, adhesion, and matrix turnover through cross-talk with TGF-β/SMAD and integrin-linked pathways. Altered RXFP1 signaling has been associated with dysregulated fibrotic responses and connective tissue homeostasis, making it relevant to studies of organ fibrosis, vascular biology, and reproductive tract physiology. As a surface receptor with context-dependent signaling bias, RXFP1 is also used to dissect GPCR desensitization, internalization, and downstream effector coupling.

Relaxin Receptor 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RXFP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RXFP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RXFP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Relaxin Receptor 1 protein expression.

This CRISPR knockout system enables efficient generation of RXFP1-deficient cell models for investigation of Relaxin Receptor 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting RXFP1 exon(s) critical for Relaxin Receptor 1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple RXFP1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Relaxin Receptor 1 CRISPR/Cas9 KO Plasmid (h) and Relaxin Receptor 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RXFP1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Relaxin Receptor 1 HDR Plasmid (h) and Relaxin Receptor 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RXFP1 homology arms to support homology-directed repair at defined RXFP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.