RelA Antibody (5G8): sc-81622
- RelA Antibody (5G8) is a mouse monoclonal IgG1 κ RelA antibody, cited in 10 publications, provided at 200 µg/ml
- raised against full length recombinant RelA of E. coli origin
- RelA Antibody (5G8) is recommended for detection of RelA of Escherichia coli origin by WB
- Anti-RelA Antibody (5G8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RelA Antibody (5G8) for WB applications. These reagents are now offered in bundles with RelA Antibody (5G8) (see ordering information below).
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RelA Antibody (5G8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the RelA protein of Escherichia coli origin by western blotting (WB). Anti-RelA antibody (5G8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. RelA plays a crucial role in the stringent response, a vital adaptive mechanism that bacteria, such as Escherichia coli, employ during periods of amino acid starvation. This response is characterized by the rapid accumulation of guanosine 3′,5′-bispyrophosphate (ppGpp), a signaling molecule that inhibits RNA synthesis, thereby reducing translation rates and facilitating the conservation of resources within the cell. RelA acts as a ppGpp synthetase, becoming activated when amino acid levels are low, and catalyzes the ATP-dependent synthesis of ppGpp. The activation of RelA occurs when uncharged tRNA molecules enter the A site of the ribosome, signaling a shortage of amino acids and prompting the synthesis of ppGpp to slow down protein production. Once amino acid levels are restored, RelA is hydrolyzed, leading to a decrease in ppGpp synthesis and the resumption of normal translation rates. This regulatory mechanism is essential for bacterial survival and adaptation to fluctuating nutrient conditions, making RelA a significant target for studies related to bacterial physiology and stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RelA Antibody (5G8) References:
- Involvement of the N terminus of ribosomal protein L11 in regulation of the RelA protein of Escherichia coli. | Yang, X. and Ishiguro, EE. 2001. J Bacteriol. 183: 6532-7. PMID: 11673421
- The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli. | Imaizumi, A., et al. 2006. J Biotechnol. 125: 328-37. PMID: 16621093
- ppGpp with DksA controls gene expression in the locus of enterocyte effacement (LEE) pathogenicity island of enterohaemorrhagic Escherichia coli through activation of two virulence regulatory genes. | Nakanishi, N., et al. 2006. Mol Microbiol. 61: 194-205. PMID: 16824105
- Identification and characterization of a second, inducible promoter of relA in Escherichia coli. | Nakagawa, A., et al. 2006. Genes Genet Syst. 81: 299-310. PMID: 17159291
- The role of the omega subunit of RNA polymerase in expression of the relA gene in Escherichia coli. | Chatterji, D., et al. 2007. FEMS Microbiol Lett. 267: 51-5. PMID: 17233676
- Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli. | Magnusson, LU., et al. 2007. J Bacteriol. 189: 5193-202. PMID: 17496080
- RelA functionally suppresses the growth defect caused by a mutation in the G domain of the essential Der protein. | Hwang, J. and Inouye, M. 2008. J Bacteriol. 190: 3236-43. PMID: 18296517
- Overexpression of the relA gene in Escherichia coli. | Schreiber, G., et al. 1991. J Biol Chem. 266: 3760-7. PMID: 1899866
- The nucleotide sequence and characterization of the relA gene of Escherichia coli. | Metzger, S., et al. 1988. J Biol Chem. 263: 15699-704. PMID: 2844820
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RelA Antibody (5G8) | sc-81622 | 200 µg/ml | $322.00 | |||
RelA Antibody (5G8): m-IgG Fc BP-HRP Bundle | sc-527061 | 200 µg Ab; 10 µg BP | $361.00 | |||
RelA Antibody (5G8): m-IgG1 BP-HRP Bundle | sc-532434 | 200 µg Ab; 20 µg BP | $361.00 | |||
RelA Antibody (5G8) AC | sc-81622 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RelA Antibody (5G8) HRP | sc-81622 HRP | 200 µg/ml | $322.00 | |||
RelA Antibody (5G8) FITC | sc-81622 FITC | 200 µg/ml | $336.00 | |||
RelA Antibody (5G8) PE | sc-81622 PE | 200 µg/ml | $349.00 | |||
RelA Antibody (5G8) Alexa Fluor® 488 | sc-81622 AF488 | 200 µg/ml | $364.00 | |||
RelA Antibody (5G8) Alexa Fluor® 546 | sc-81622 AF546 | 200 µg/ml | $364.00 | |||
RelA Antibody (5G8) Alexa Fluor® 594 | sc-81622 AF594 | 200 µg/ml | $364.00 | |||
RelA Antibody (5G8) Alexa Fluor® 647 | sc-81622 AF647 | 200 µg/ml | $364.00 | |||
RelA Antibody (5G8) Alexa Fluor® 680 | sc-81622 AF680 | 200 µg/ml | $364.00 | |||
RelA Antibody (5G8) Alexa Fluor® 790 | sc-81622 AF790 | 200 µg/ml | $364.00 |