Reg IIIγ CRISPR/Cas9 KO Plasmid (h): sc-418109
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Reg IIIγ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Reg IIIγ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Reg IIIγ Antibody (179A2Y): sc-517630
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Reg IIIγ CRISPR/Cas9 KO Plasmid (h) | sc-418109 | 20 µg | $397.00 |
Overview
REG3G encodes regenerating islet-derived protein 3 gamma (Reg IIIγ), a secreted C-type lectin–like antimicrobial protein implicated in epithelial barrier defense and innate immune regulation at mucosal surfaces. Reg IIIγ recognizes bacterial cell wall glycans and contributes to controlling microbial colonization, linking its activity to host–microbiome interactions and inflammatory signaling in the gastrointestinal tract. Expression is commonly inducible by cytokine-driven pathways, including IL-22/STAT3 signaling, and can be modulated downstream of pattern-recognition receptor activation and NF-κB–associated responses. Dysregulated REG3G expression has been associated with altered mucosal immunity and inflammation-relevant phenotypes, supporting its use as a functional node in studies of barrier dysfunction and immune-mediated disease mechanisms.
Reg IIIγ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the REG3G gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the REG3G together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the REG3G open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Reg IIIγ protein expression.
This CRISPR knockout system enables efficient generation of REG3G-deficient cell models for investigation of Reg IIIγ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting REG3G exon(s) critical for Reg IIIγ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple REG3G genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Reg IIIγ CRISPR/Cas9 KO Plasmid (h) and Reg IIIγ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the REG3G locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Reg IIIγ HDR Plasmid (h) and Reg IIIγ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by REG3G homology arms to support homology-directed repair at defined REG3G target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.