Reg IIIα CRISPR/Cas9 KO Plasmid (h): sc-404278
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Reg IIIα CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Reg IIIα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Reg IIIα CRISPR/Cas9 KO Plasmid (h) | sc-404278 | 20 µg | $397.00 |
Overview
REG3A encodes the secreted lectin Reg IIIα, a C-type lectin family antimicrobial protein expressed predominantly by epithelial cells in the gastrointestinal tract and other mucosal surfaces. Reg IIIα binds microbial cell wall carbohydrates, supports innate barrier defense, and contributes to host–microbiota homeostasis during inflammation. Its expression is regulated by cytokine-driven epithelial programs, including IL-22/STAT3 signaling and NF-κB–associated responses, and is frequently used as a readout of mucosal injury and repair. Dysregulated REG3A levels have been reported in inflammatory and metabolic conditions affecting barrier tissues, making it relevant for studies of epithelial stress responses and microbe-triggered inflammation.
Reg IIIα CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the REG3A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the REG3A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the REG3A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Reg IIIα protein expression.
This CRISPR knockout system enables efficient generation of REG3A-deficient cell models for investigation of Reg IIIα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting REG3A exon(s) critical for Reg IIIα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple REG3A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Reg IIIα CRISPR/Cas9 KO Plasmid (h) and Reg IIIα CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the REG3A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Reg IIIα HDR Plasmid (h) and Reg IIIα HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by REG3A homology arms to support homology-directed repair at defined REG3A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.