REEP6 CRISPR/Cas9 KO Plasmid (m): sc-427671
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- REEP6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the REEP6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: REEP6 Antibody (H-9): sc-393569
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REEP6 CRISPR/Cas9 KO Plasmid (m) | sc-427671 | 20 µg | $397.00 |
Overview
Reep6 encodes receptor expression-enhancing protein 6 (REEP6), a member of the REEP family implicated in shaping the tubular endoplasmic reticulum and coordinating protein trafficking within the early secretory pathway. In mouse cells, REEP6 is associated with membrane curvature and the organization of ER subdomains that support efficient processing and transport of transmembrane proteins, with downstream effects on cellular homeostasis and stress responses. REEP6 is highly relevant to studies of neuronal and sensory biology, as REEP-family proteins have been linked to processes that maintain specialized membrane compartments and proteostasis. Dysregulation of ER architecture and trafficking pathways involving REEP proteins is frequently explored in the context of neurodegeneration and sensory system dysfunction, supporting mechanistic investigations in relevant models.
REEP6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Reep6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Reep6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Reep6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish REEP6 protein expression.
This CRISPR knockout system enables efficient generation of Reep6-deficient cell models for investigation of REEP6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Reep6 exon(s) critical for REEP6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Reep6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by REEP6 CRISPR/Cas9 KO Plasmid (m) and REEP6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Reep6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by REEP6 HDR Plasmid (m) and REEP6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Reep6 homology arms to support homology-directed repair at defined Reep6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.