Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RecQL4 Double Nickase Plasmid (m): sc-429775-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RecQL4 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RecQL4 Double Nickase Plasmid (m) and RecQL4 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Recql4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RecQL4 Antibody (B-3): sc-518189
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RecQL4 Double Nickase Plasmid (m) | sc-429775-NIC | 20 µg | $410.00 |
Mouse Recql4 encodes the RecQL4 helicase, a member of the RecQ family that supports genome maintenance during DNA replication and repair. RecQL4 participates in replication origin firing and DNA end resection and contributes to pathways including homologous recombination, non-homologous end joining, and base excision repair to limit replication stress and chromosomal instability. Loss or dysfunction of RECQL4 is linked to inherited genome instability syndromes and cancer predisposition phenotypes, making it a useful node for studying DNA damage signaling, checkpoint control, and replication-associated break repair. Recql4 perturbation is commonly used to interrogate how helicase-driven fork restart and repair pathway choice influence cell fate under genotoxic stress.
RecQL4 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Recql4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Recql4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Recql4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Recql4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.