RCK Antibody (YZ-14): sc-101019
- RCK Antibody (YZ-14) is a mouse monoclonal mouse IgG1 κ κ provided at 100 µg/ml
- raised against recombinant RCK of human origin
- recommended for detection of RCK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RCK Antibody (YZ-14). This work is in progress.
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RCK Antibody (YZ-14) is a mouse monoclonal IgG1 antibody that detects RCK in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RCK, also known as DDX6 or P54, is a crucial member of the DEAD-box RNA helicase family, characterized by common protein motifs and widespread presence in various tissues. RCK (YZ-14) antibody recognizes a protein that plays a vital role in RNA metabolism, functioning as an unwindase that exhibits ATP-dependent RNA unwinding activity and facilitates RNA decay in the 5′-3′ direction. Anti-RCK antibody (YZ-14) targets a protein integral to normal cellular processes such as splicing, RNA export, and translation initiation, ensuring proper regulation of gene expression. Notably, RCK′s unwindase activity is essential for downregulating mRNA expression, which helps maintain normal transcriptional levels within cells. However, mutations in the gene encoding RCK can lead to overexpression, transforming RCK into an oncogene that promotes the expression of genes associated with cell growth and proliferation. Understanding RCK function and regulation is critical, as this highlights the delicate balance of RNA metabolism and implications in cellular health and disease.
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RCK Antibody (YZ-14) References:
- Overexpression of rck/p54, a DEAD box protein, in human colorectal tumours. | Nakagawa, Y., et al. 1999. Br J Cancer. 80: 914-7. PMID: 10360675
- Expression of rck/p54, a DEAD-box RNA helicase, in gametogenesis and early embryogenesis of mice. | Matsumoto, K., et al. 2005. Dev Dyn. 233: 1149-56. PMID: 15906376
- Structures of the MthK RCK domain and the effect of Ca2+ on gating ring stability. | Dong, J., et al. 2005. J Biol Chem. 280: 41716-24. PMID: 16227203
- The RCK domain of the KtrAB K+ transporter: multiple conformations of an octameric ring. | Albright, RA., et al. 2006. Cell. 126: 1147-59. PMID: 16990138
- Human complement factor H binds to outer membrane protein Rck of Salmonella. | Ho, DK., et al. 2010. J Immunol. 185: 1763-9. PMID: 20622116
- Role of Rck-Pat1b binding in assembly of processing-bodies. | Ozgur, S. and Stoecklin, G. 2013. RNA Biol. 10: 528-39. PMID: 23535175
- Free RCK arrangement in Kch, a putative escherichia coli potassium channel, as suggested by electron crystallography. | Kuang, Q., et al. 2015. Structure. 23: 199-205. PMID: 25497729
- RCK: accurate and efficient inference of sequence- and structure-based protein-RNA binding models from RNAcompete data. | Orenstein, Y., et al. 2016. Bioinformatics. 32: i351-i359. PMID: 27307637
- How RCK domains regulate gating of K+ channels. | Schrecker, M., et al. 2019. Biol Chem. 400: 1303-1322. PMID: 31361596
- Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site. | Teixeira-Duarte, CM., et al. 2019. Elife. 8: PMID: 31868587
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RCK Antibody (YZ-14) | sc-101019 | 100 µg/ml | $339.00 |