RCC1 Double Nickase Plasmid (h): sc-402078-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RCC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RCC1 Double Nickase Plasmid (h) and RCC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RCC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RCC1 Antibody (E-6): sc-55559
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RCC1 Double Nickase Plasmid (h) | sc-402078-NIC | 20 µg | $410.00 | |||
RCC1 Double Nickase Plasmid (h2) | sc-402078-NIC-2 | 20 µg | $410.00 |
RCC1 encodes regulator of chromosome condensation 1, a chromatin-bound guanine nucleotide exchange factor that activates Ran by promoting GDP-to-GTP exchange. The resulting RanGTP gradient coordinates nucleocytoplasmic transport, spindle assembly, and nuclear envelope reformation, linking RCC1 to cell cycle progression and genome stability. RCC1 function is integrated with mitotic checkpoint control and chromatin dynamics, and perturbation can alter chromosome segregation and DNA damage responses. Dysregulated RCC1–Ran signaling has been associated with proliferative phenotypes and is frequently studied in the context of cancer biology and mitotic stress pathways.
RCC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RCC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RCC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RCC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RCC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.