RBM4 Antibody (E-3): sc-373852

RBM4 Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RBM4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RBM4 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RBM4 protein, also known as RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, or ZCCHC21, is a 364 amino acid protein that plays a crucial role in the regulation of alternative splicing during pre-mRNA processing. RBM4 is expressed ubiquitously and is localized in both the nucleus and cytoplasm, where RBM4 contains one CCHC-type zinc finger and two RNA recognition motifs (RRM). The presence of these structural features facilitates RBM4′s interaction with various RNA molecules, thereby influencing gene expression and cellular function. RBM4 interacts with Importin-12, which is essential for nuclear import, highlighting RBM4′s importance in cellular transport mechanisms. RBM4 has been implicated in brain development, as RBM4 is down-regulated in patients with fetal Down syndrome, suggesting a potential role in normal neurodevelopmental processes. Multiple isoforms of RBM4 exist due to alternative splicing, with RBM4B (also known as RBM30) being a closely related protein that similarly regulates alternative splicing.

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RBM4 Antibody (E-3) References:

1. Manifold decreased protein levels of matrin 3, reduced motor protein HMP and hlark in fetal Down's syndrome brain.  |  Bernert, G., et al. 2002. Proteomics. 2: 1752-7. PMID: 12469345
2. Exon selection in alpha-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB.  |  Lin, JC. and Tarn, WY. 2005. Mol Cell Biol. 25: 10111-21. PMID: 16260624
3. RBM4 interacts with an intronic element and stimulates tau exon 10 inclusion.  |  Kar, A., et al. 2006. J Biol Chem. 281: 24479-88. PMID: 16777844
4. WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo.  |  Markus, MA., et al. 2006. Exp Cell Res. 312: 3379-88. PMID: 16934801
5. Cell stress modulates the function of splicing regulatory protein RBM4 in translation control.  |  Lin, JC., et al. 2007. Proc Natl Acad Sci U S A. 104: 2235-40. PMID: 17284590
6. The LARK/RBM4a protein is highly expressed in cerebellum as compared to cerebrum.  |  Pfuhl, T., et al. 2008. Neurosci Lett. 444: 11-5. PMID: 18708123
7. Multiple roles of RBM4 in muscle cell differentiation.  |  Lin, JC. and Tarn, WY. 2012. Front Biosci (Schol Ed). 4: 181-9. PMID: 22202052
8. Nuclear Aurora kinase A switches m6A reader YTHDC1 to enhance an oncogenic RNA splicing of tumor suppressor RBM4.  |  Li, S., et al. 2022. Signal Transduct Target Ther. 7: 97. PMID: 35361747
9. RBM4 inhibits the growth of clear cell renal cell carcinoma by enhancing the stability of p53 mRNA.  |  Jian, W., et al. 2023. Mol Carcinog. 62: 464-478. PMID: 36585906
10. RBM4 regulates cellular senescence via miR1244/SERPINE1 axis.  |  Wang, L., et al. 2023. Cell Death Dis. 14: 27. PMID: 36639375
11. RBM4 dictates ESCC cell fate switch from cellular senescence to glutamine-addiction survival through inhibiting LKB1-AMPK-axis.  |  Chen, L., et al. 2023. Signal Transduct Target Ther. 8: 159. PMID: 37080995
12. A novel zinc finger-containing RNA-binding protein conserved from fruitflies to humans.  |  Jackson, FR., et al. 1997. Genomics. 41: 444-52. PMID: 9169144

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RBM4 Antibody (E-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-RBM4 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RBM4 Antibody (E-3) Specifications

Alternate Product Name: RBM4

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-100

Epitope Target: RBM4

Epitope Species: human

Product Reactivity/Detection: RBM4A and RBM4B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for RBM4 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin