RBM15 CRISPR/Cas9 KO Plasmid (m): sc-432890
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBM15 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RBM15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM15 CRISPR/Cas9 KO Plasmid (m) | sc-432890 | 20 µg | $397.00 |
Overview
Rbm15 encodes RBM15, an RNA-binding protein that participates in post-transcriptional gene regulation, including alternative splicing, mRNA export, and stability control. RBM15 is linked to epitranscriptomic regulation through interactions with m6A writer-associated complexes, shaping cell state transitions and lineage-specific gene expression programs. In mouse cells, RBM15 has been implicated in hematopoietic and developmental processes where coordinated RNA processing is required for differentiation and proliferation. Dysregulation of RBM15-associated RNA regulatory networks is studied in the context of oncogenic transformation and aberrant differentiation phenotypes, supporting mechanistic work in RNA biology and disease models.
RBM15 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rbm15 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rbm15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rbm15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RBM15 protein expression.
This CRISPR knockout system enables efficient generation of Rbm15-deficient cell models for investigation of RBM15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rbm15 exon(s) critical for RBM15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rbm15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RBM15 CRISPR/Cas9 KO Plasmid (m) and RBM15 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rbm15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RBM15 HDR Plasmid (m) and RBM15 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rbm15 homology arms to support homology-directed repair at defined Rbm15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.