RbAp46/p48 Antibody (G-8): sc-373873

RbAp46/p48 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects RbAp46/p48 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RbAp46/p48 antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RbAp46/p48 plays a crucial role in chromatin remodeling, which is essential for gene expression regulation. Located primarily in the nucleus, RbAp46 and RbAp48 are integral components of the histone deacetylase complex, where RbAp46 and RbAp48 interact with histones and other nuclear proteins to modulate chromatin structure. This interaction is vital because histone acetylation and deacetylation directly influence DNA accessibility to transcription factors, thereby impacting transcriptional activity. RbAp46/p48 binding to acetylated histones allows for regulatory protein recruitment, making RbAp46/p48 a key player in dynamic transcriptional regulation. Understanding RbAp46/p48 function and role in chromatin dynamics is important for elucidating mechanisms underlying gene expression and implications in various biological processes and diseases.

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RbAp46/p48 Antibody (G-8) References:

1. The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein.  |  Nicolas, E., et al. 2001. Nucleic Acids Res. 29: 3131-6. PMID: 11470869
2. Radiation-inducible protein RbAp48 contributes to radiosensitivity of cervical cancer cells.  |  Zheng, L., et al. 2013. Gynecol Oncol. 130: 601-8. PMID: 23756179
3. Structure Based Design of Bicyclic Peptide Inhibitors of RbAp48.  |  Hart, P'., et al. 2021. Angew Chem Int Ed Engl. 60: 1813-1820. PMID: 33022847
4. Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.  |  Qian, YW. and Lee, EY. 1995. J Biol Chem. 270: 25507-13. PMID: 7503932
5. Nucleosome structural changes due to acetylation.  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
6. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
7. Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation.  |  Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
8. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p.  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
9. A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A.  |  Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
10. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3.  |  Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
11. Identification of mouse histone deacetylase 1 as a growth factor-inducible gene.  |  Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381
12. Characterization of a human RPD3 ortholog, HDAC3.  |  Emiliani, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2795-800. PMID: 9501169

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RbAp46/p48 Antibody (G-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-RbAp46/p48 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RbAp46/p48 Antibody (G-8) Specifications

Alternate Product Name: RbAp46/p48

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 126-425

Epitope Target: RbAp48

Epitope Species: human

Product Reactivity/Detection: RbAp46 and RbAp48

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for RbAp46/p48 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred RbAp46 antibody is RbAp46 Antibody (E-9): sc-377197.