Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RbAp46 CRISPR/Cas9 KO Plasmid (h): sc-405076
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RbAp46 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RbAp46 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RbAp46 Antibody (E-9): sc-377197
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RbAp46 CRISPR/Cas9 KO Plasmid (h) | sc-405076 | 20 µg | $397.00 |
Overview
RBBP7 encodes RbAp46, a WD40 repeat histone-binding protein that functions as a core component of multiple chromatin regulatory complexes, including NuRD/HDAC, Sin3A, and PRC2, and associates with CAF-1 during nucleosome assembly. Through these interactions, RbAp46 helps coordinate histone deacetylation, chromatin remodeling, and epigenetic gene silencing, impacting transcriptional programs, DNA replication-coupled chromatin assembly, and genome stability. Altered RBBP7-dependent chromatin regulation has been linked to dysregulated proliferation, differentiation defects, and stress-response phenotypes observed across cancer and developmental disease contexts. As a nodal chromatin factor, RbAp46 is frequently studied in pathways controlling cell cycle progression, DNA damage responses, and lineage-specific transcription.
RbAp46 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RBBP7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RBBP7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RBBP7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RbAp46 protein expression.
This CRISPR knockout system enables efficient generation of RBBP7-deficient cell models for investigation of RbAp46 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RBBP7 exon(s) critical for RbAp46 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RBBP7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RbAp46 CRISPR/Cas9 KO Plasmid (h) and RbAp46 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RBBP7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RbAp46 HDR Plasmid (h) and RbAp46 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RBBP7 homology arms to support homology-directed repair at defined RBBP7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.