Rb (h2): 293T Lysate: sc-159907

Rb (h2): 293T Lysate is sourced from the human embryonic kidney 293T cell line, genetically modified to express the human retinoblastoma protein (Rb). Rb is a pivotal tumor suppressor protein involved in regulating the cell cycle, particularly the transition from the G1 to the S phase. The 293T cell line, noted for its high transfection efficiency and robust protein expression, is ideal for producing Rb protein lysates. In research, Rb (h2): 293T Lysate is extensively used to explain the molecular mechanisms governing cell cycle control and tumor suppression. Mechanistically, Rb functions by binding to and inhibiting E2F transcription factors, thereby repressing the transcription of genes essential for DNA replication. This inhibitory action is modulated through phosphorylation by cyclin-dependent kinases (CDKs), which inactivate Rb and release E2F. Researchers employ Rb (h2): 293T Lysate in various assays, including Western blotting, immunoprecipitation, and kinase assays, to investigate the phosphorylation status of Rb and its interactions with other cell cycle regulators. Furthermore, this lysate is instrumental in chromatin immunoprecipitation (ChIP) assays to explore Rb's role in gene repression and epigenetic regulation. The lysate is also utilized in functional studies to dissect the pathways involved in Rb-mediated growth inhibition and to identify novel protein interactions, advancing our understanding of cell cycle dynamics and oncogenesis.