Rb (h): 293T Lysate: sc-114014

Rb (h): 293T Lysate is derived from the human embryonic kidney 293T cell line, engineered to express the human retinoblastoma protein (Rb). Rb is a crucial tumor suppressor protein that regulates the cell cycle by controlling the transition from the G1 phase to the S phase. The 293T cell line, known for its high transfection efficiency and protein expression capabilities, is ideal for producing Rb protein lysates. In research, Rb (h): 293T Lysate is utilized to study the molecular mechanisms underlying cell cycle regulation and tumor suppression. Rb functions by binding to E2F transcription factors, inhibiting their activity, and preventing the transcription of genes required for DNA replication. This interaction is regulated through phosphorylation by cyclin-dependent kinases (CDKs). Researchers use Rb (h): 293T Lysate in various assays, including Western blotting, immunoprecipitation, and kinase assays, to investigate the phosphorylation status of Rb and its interactions with other cell cycle regulators. Additionally, this lysate is valuable in chromatin immunoprecipitation (ChIP) assays to explore Rb's role in gene repression. The lysate is also employed in functional studies to dissect the pathways involved in Rb-mediated growth inhibition and to identify novel proteins that interact with Rb, advancing our understanding of cell cycle control and tumorigenesis.