rapsyn CRISPR/Cas9 KO Plasmid (m): sc-422599
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- rapsyn CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the rapsyn genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: rapsyn Antibody (1234): sc-58585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
rapsyn CRISPR/Cas9 KO Plasmid (m) | sc-422599 | 20 µg | $397.00 |
Overview
Rapsn encodes rapsyn, a postsynaptic scaffolding protein that anchors and clusters nicotinic acetylcholine receptors at the neuromuscular junction, coordinating synapse maturation and maintenance. Through interactions with AChR subunits and cytoskeletal components, rapsyn supports receptor stabilization, endplate organization, and efficient neuromuscular transmission. Disruption of Rapsn-dependent clustering perturbs synaptic architecture and signaling at the motor endplate, linking this pathway to neuromuscular junction dysfunction. Mouse Rapsn is therefore widely used to model mechanisms that govern synaptogenesis, receptor trafficking, and synaptic stability in skeletal muscle.
rapsyn CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rapsn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rapsn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rapsn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish rapsyn protein expression.
This CRISPR knockout system enables efficient generation of Rapsn-deficient cell models for investigation of rapsyn signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rapsn exon(s) critical for rapsyn function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rapsn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by rapsyn CRISPR/Cas9 KO Plasmid (m) and rapsyn CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rapsn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by rapsyn HDR Plasmid (m) and rapsyn HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rapsn homology arms to support homology-directed repair at defined Rapsn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.