RAP55 Antibody (C-5): sc-398552
- RAP55 Antibody (C-5) is a mouse monoclonal IgG1 κ RAP55 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 103-197 mapping within an internal region of RAP55 of human origin
- RAP55 Antibody (C-5) is recommended for detection of RAP55 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RAP55 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RAP55 Antibody (C-5) for WB applications. These reagents are now offered in bundles with RAP55 Antibody (C-5) (see ordering information below).
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RAP55 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects RAP55 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RAP55 Antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RAP55, also known as LSM14A, is a 463 amino acid member of the LSM14 family characterized by its FFD-TFG box domain, which plays a crucial role in RNA metabolism and regulation. RAP55 is involved in the formation of processing bodies (P-bodies), where RAP55 accumulates due to the presence of the LSM14 domain and RGG repeats, essential for mRNA decay and storage. The FDF motif within RAP55 is critical for cytoplasmic retention, ensuring RAP55 remains in the appropriate cellular compartment to perform its functions. RAP55 undergoes phosphorylation in response to DNA damage, likely mediated by ATM or ATR kinases, highlighting RAP55′s role in cellular stress responses. The gene encoding RAP55 is conserved across a wide range of species, including chimpanzees, dogs, cows, and various model organisms, and is located on human chromosome 19q13.11. This conservation underscores RAP55′s fundamental importance in cellular processes, making anti-RAP55 antibody (C-5) a valuable tool for researchers studying RNA metabolism and cellular responses to stress.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RAP55 Antibody (C-5) References:
- RNA-associated protein 55 (RAP55) localizes to mRNA processing bodies and stress granules. | Yang, WH., et al. 2006. RNA. 12: 547-54. PMID: 16484376
- RAP55, a cytoplasmic mRNP component, represses translation in Xenopus oocytes. | Tanaka, KJ., et al. 2006. J Biol Chem. 281: 40096-106. PMID: 17074753
- ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. | Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
- A quantitative atlas of mitotic phosphorylation. | Dephoure, N., et al. 2008. Proc Natl Acad Sci U S A. 105: 10762-7. PMID: 18669648
- RAP55: insights into an evolutionarily conserved protein family. | Marnef, A., et al. 2009. Int J Biochem Cell Biol. 41: 977-81. PMID: 18723115
- Identification of a novel mRNA-associated protein in oocytes of Pleurodeles waltl and Xenopus laevis. | Lieb, B., et al. 1998. Exp Cell Res. 245: 272-81. PMID: 9851867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RAP55 Antibody (C-5) | sc-398552 | 200 µg/ml | $322.00 | |||
RAP55 Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-527785 | 200 µg Ab; 10 µg BP | $361.00 | |||
RAP55 Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-533158 | 200 µg Ab; 20 µg BP | $361.00 | |||
RAP55 Antibody (C-5) AC | sc-398552 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RAP55 Antibody (C-5) HRP | sc-398552 HRP | 200 µg/ml | $322.00 | |||
RAP55 Antibody (C-5) FITC | sc-398552 FITC | 200 µg/ml | $336.00 | |||
RAP55 Antibody (C-5) PE | sc-398552 PE | 200 µg/ml | $349.00 | |||
RAP55 Antibody (C-5) Alexa Fluor® 488 | sc-398552 AF488 | 200 µg/ml | $364.00 | |||
RAP55 Antibody (C-5) Alexa Fluor® 546 | sc-398552 AF546 | 200 µg/ml | $364.00 | |||
RAP55 Antibody (C-5) Alexa Fluor® 594 | sc-398552 AF594 | 200 µg/ml | $364.00 | |||
RAP55 Antibody (C-5) Alexa Fluor® 647 | sc-398552 AF647 | 200 µg/ml | $364.00 | |||
RAP55 Antibody (C-5) Alexa Fluor® 680 | sc-398552 AF680 | 200 µg/ml | $364.00 | |||
RAP55 Antibody (C-5) Alexa Fluor® 790 | sc-398552 AF790 | 200 µg/ml | $364.00 |