Rap1GAP Antibody (E-11): sc-390826

Rap1GAP Antibody (E-11) is a mouse monoclonal IgG1 antibody that detects Rap1GAP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rap1GAP plays a crucial role in cellular signaling by stimulating the GTP hydrolytic activity of the monomeric G protein Rap1, which is essential for regulating various cellular processes, including cell proliferation and differentiation. Rap1GAP is primarily located in the brain, kidney, and pancreas, where Rap1GAP acts as a signal integrator, coordinating Gz signaling and Rap1 signaling pathways. Notably, Rap1GAP interacts with Gα z, a member of the Gi family of trimeric G proteins, which inhibits the ability of regulators of G protein signaling to promote GTP hydrolysis of the α subunit, thereby modulating downstream signaling events. Additionally, a novel isoform known as Rap1GAPII has been identified, which specifically binds to Gα z and is translocated from the cytosol to the membrane upon stimulation of the Gi-coupled M2 muscarinic receptor, leading to a decrease in GTP-bound Rap1 and subsequent activation of the ERK/MAPK pathway. anti-Rap1GAP antibody (E-11) is an invaluable tool for researchers studying the intricate mechanisms of G protein signaling and its implications in various physiological and pathological processes.

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Rap1GAP Antibody (E-11) References:

1. Thyroid-stimulating hormone/cAMP and glycogen synthase kinase 3beta elicit opposing effects on Rap1GAP stability.  |  Tsygankova, OM., et al. 2004. J Biol Chem. 279: 5501-7. PMID: 14660640
2. Down-regulation of Rap1GAP via promoter hypermethylation promotes melanoma cell proliferation, survival, and migration.  |  Zheng, H., et al. 2009. Cancer Res. 69: 449-57. PMID: 19147557
3. Inhibitory effects of Rap1GAP overexpression on proliferation and migration of endothelial cells via ERK and Akt pathways.  |  Li, W., et al. 2011. J Huazhong Univ Sci Technolog Med Sci. 31: 721-727. PMID: 22173489
4. Rap1GAP regulates renal cell carcinoma invasion.  |  Kim, WJ., et al. 2012. Cancer Lett. 320: 65-71. PMID: 22266190
5. Tumor cell migration and invasion are enhanced by depletion of Rap1 GTPase-activating protein (Rap1GAP).  |  Tsygankova, OM., et al. 2013. J Biol Chem. 288: 24636-46. PMID: 23864657
6. Podocyte-specific RAP1GAP expression contributes to focal segmental glomerulosclerosis-associated glomerular injury.  |  Potla, U., et al. 2014. J Clin Invest. 124: 1757-69. PMID: 24642466
7. RAP1GAP Functions as a Tumor Suppressor Gene and Is Regulated by DNA Methylation in Differentiated Thyroid Cancer.  |  Faam, B., et al. 2021. Cytogenet Genome Res. 161: 227-235. PMID: 34311462
8. Simulated Microgravity Increases the Permeability of HUVEC Monolayer through Up-Regulation of Rap1GAP and Decreased Rap2 Activation.  |  Shi, S., et al. 2022. Int J Mol Sci. 23: PMID: 35054818
9. Evaluation of Rap1GAP and EPAC1 Gene Expression in Endometriosis Disease.  |  Dehghanian, M., et al. 2023. Adv Biomed Res. 12: 101. PMID: 37288024
10. Phosphorylation of Rap1GAP during the cell cycle.  |  Janoueix-Lerosey, I., et al. 1994. Biochem Biophys Res Commun. 202: 967-75. PMID: 8048970

The preferred Rap1GAP antibody is Rap1GAP Antibody (D-9): sc-166586.