Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rap 2A CRISPR/Cas9 KO Plasmid (h): sc-403109
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rap 2A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rap 2A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rap 2A CRISPR/Cas9 KO Plasmid (h) | sc-403109 | 20 µg | $397.00 |
Overview
RAP2A encodes Rap2A, a Ras-related small GTPase that cycles between GDP- and GTP-bound states to regulate signal transduction downstream of cell-surface receptors. Rap2A contributes to cytoskeletal remodeling, vesicle trafficking, and cell adhesion by coordinating pathways that influence actin dynamics and membrane organization, including crosstalk with MAPK signaling and Rho-family GTPase networks. Through its roles in controlling cell polarity, migration, and proliferation, altered RAP2A activity has been linked to dysregulated growth and invasion phenotypes observed in multiple disease contexts, including cancer and inflammatory conditions. RAP2A is therefore a useful node for dissecting how small GTPase circuits tune receptor-driven signaling and cellular plasticity.
Rap 2A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAP2A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAP2A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAP2A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rap 2A protein expression.
This CRISPR knockout system enables efficient generation of RAP2A-deficient cell models for investigation of Rap 2A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAP2A exon(s) critical for Rap 2A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAP2A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rap 2A CRISPR/Cas9 KO Plasmid (h) and Rap 2A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAP2A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rap 2A HDR Plasmid (h) and Rap 2A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAP2A homology arms to support homology-directed repair at defined RAP2A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.