Ran BP-M Antibody (F-1): sc-271727

Ran BP-M Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ran BP-M of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Ran BP-M (F-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ran BP-M, also known as Ran-binding protein 9, plays a crucial role in the nucleation of microtubule networks and serves as an adapter protein that links membrane receptors to intracellular signaling pathways. This function facilitates the transport of proteins and RNA through the nuclear pore complex, thereby influencing key cellular processes such as DNA replication and mitosis. Ran BP-M is involved in the activation of androgen and glucocorticoid receptors, which are vital for various hormonal signaling pathways. Phosphorylated serine residues characterize Ran BP-M, which can affect localization and function; for instance, a phosphorylated form can associate with the plasma membrane, highlighting a dynamic role in cellular signaling. Ran BP-M shows highest expression levels in heart, testis, muscle, and placenta, and enzymatic activity has been linked to breast cancer progression and fragile-X mental retardation, underscoring significance in both normal physiology and disease states.

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Ran BP-M Antibody (F-1) References:

1. RanBPM, a nuclear protein that interacts with and regulates transcriptional activity of androgen receptor and glucocorticoid receptor.  |  Rao, MA., et al. 2002. J Biol Chem. 277: 48020-7. PMID: 12361945
2. RanBPM is a phosphoprotein that associates with the plasma membrane and interacts with the integrin LFA-1.  |  Denti, S., et al. 2004. J Biol Chem. 279: 13027-34. PMID: 14722085
3. Protein stability and function of p73 are modulated by a physical interaction with RanBPM in mammalian cultured cells.  |  Kramer, S., et al. 2005. Oncogene. 24: 938-44. PMID: 15558019
4. The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif.  |  Beddow, AL., et al. 1995. Proc Natl Acad Sci U S A. 92: 3328-32. PMID: 7724562
5. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form.  |  Scheffzek, K., et al. 1995. Nature. 374: 378-81. PMID: 7885480
6. Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.  |  Ren, M., et al. 1995. Mol Cell Biol. 15: 2117-24. PMID: 7891706
7. When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic microtubule nucleation similar to gamma-tubulin.  |  Nakamura, M., et al. 1998. J Cell Biol. 143: 1041-52. PMID: 9817760

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ran BP-M Antibody (F-1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Ran BP-M Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ran BP-M Antibody (F-1) Specifications

Alternate Product Name: Ran BP-M

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 517-563

Epitope Target: Ran BP-M

Epitope Species: human

Product Reactivity/Detection: Ran BP-M isoform 1 and 2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Ran BP-M Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin