Ran BP-3 Double Nickase Plasmid (h): sc-405001-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ran BP-3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ran BP-3 Double Nickase Plasmid (h) and Ran BP-3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RANBP3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ran BP-3 Antibody (D-2): sc-377253
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ran BP-3 Double Nickase Plasmid (h) | sc-405001-NIC | 20 µg | $410.00 | |||
Ran BP-3 Double Nickase Plasmid (h2) | sc-405001-NIC-2 | 20 µg | $410.00 |
RANBP3 encodes Ran-binding protein 3 (Ran BP-3), a nuclear export cofactor that participates in RanGTP-dependent nucleocytoplasmic transport. Ran BP-3 interacts with export receptors to support CRM1/XPO1-mediated export and contributes to maintaining proper nuclear–cytoplasmic distribution of regulatory proteins and RNAs. Through its role in transport dynamics, RANBP3 interfaces with pathways controlling transcriptional programs, cell-cycle progression, and stress responses. Dysregulation of nuclear export machinery, including factors that modulate Ran and exportin function, is implicated in altered signaling and genome-stability phenotypes relevant to cancer and other proliferative or neurodegenerative contexts.
Ran BP-3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RANBP3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RANBP3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RANBP3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RANBP3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.