Ran BP-2 CRISPR/Cas9 KO Plasmid (m): sc-422596
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ran BP-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ran BP-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ran BP-2 Antibody (D-4): sc-74518
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ran BP-2 CRISPR/Cas9 KO Plasmid (m) | sc-422596 | 20 µg | $397.00 |
Overview
Ranbp2 encodes Ran BP-2 (Nup358), a major cytoplasmic filament component of the nuclear pore complex that coordinates Ran GTPase–dependent nucleocytoplasmic transport and contributes to nuclear envelope homeostasis. As a SUMO E3 ligase, it regulates SUMOylation of select cargoes and participates in processes including mitotic progression, microtubule dynamics, and proteostasis. Ran BP-2 also interfaces with pathways governing mRNA export and stress responses, linking nuclear transport to cell-cycle control and cellular adaptation. Dysregulation of nucleocytoplasmic trafficking and SUMO-dependent regulation has been associated with neurodegenerative phenotypes and altered immune signaling, making Ranbp2 a useful node for mechanistic studies in mouse systems.
Ran BP-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ranbp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ranbp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ranbp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ran BP-2 protein expression.
This CRISPR knockout system enables efficient generation of Ranbp2-deficient cell models for investigation of Ran BP-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ranbp2 exon(s) critical for Ran BP-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ranbp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ran BP-2 CRISPR/Cas9 KO Plasmid (m) and Ran BP-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ranbp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ran BP-2 HDR Plasmid (m) and Ran BP-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ranbp2 homology arms to support homology-directed repair at defined Ranbp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.