RAI15 CRISPR/Cas9 KO Plasmid (h): sc-405878
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RAI15 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RAI15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RAI15 CRISPR/Cas9 KO Plasmid (h) | sc-405878 | 20 µg | $397.00 |
Overview
SMYD5 encodes a SET and MYND domain–containing lysine methyltransferase implicated in chromatin regulation and transcriptional control, supporting context-dependent programs that shape cell identity and stress responses. Through modulation of histone and protein methylation, SMYD5 is linked to epigenetic pathways that influence DNA-templated processes, including replication-coupled chromatin assembly and gene expression dynamics. Dysregulated SMYD5 activity has been associated with altered inflammatory signaling and oncogenic transcriptional states in multiple model systems, making it relevant for dissecting mechanisms of tumor progression and immune-related phenotypes. In human cells, interrogation of SMYD5-dependent networks can clarify how epigenetic modifiers integrate signaling cues to regulate proliferation and differentiation.
RAI15 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SMYD5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SMYD5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SMYD5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RAI15 protein expression.
This CRISPR knockout system enables efficient generation of SMYD5-deficient cell models for investigation of RAI15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SMYD5 exon(s) critical for RAI15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SMYD5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RAI15 CRISPR/Cas9 KO Plasmid (h) and RAI15 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SMYD5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RAI15 HDR Plasmid (h) and RAI15 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SMYD5 homology arms to support homology-directed repair at defined SMYD5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.