Raftlin-2 Antibody (F-7): sc-514540
- Raftlin-2 Antibody (F-7) is a mouse monoclonal IgG1 κ Raftlin-2 antibody provided at 200 µg/ml
- raised against amino acids 32-211 mapping near the N-terminus of Raftlin-2 of human origin
- Raftlin-2 Antibody (F-7) is recommended for detection of Raftlin-2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Raftlin-2 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Raftlin-2 Antibody (F-7) for WB applications. This reagent is now offered in a bundle with Raftlin-2 Antibody (F-7) (see ordering information below).
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Raftlin-2 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Raftlin-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Raftlin-2 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Raftlin-2, also known as RFTN2 (raftlin family member 2), is a crucial 501 amino acid cell membrane protein that plays a significant role in the assembly and maintenance of lipid rafts, which are specialized membrane microdomains essential for B-cell activation during B-cell receptor signal initiation. Proper functioning of Raftlin-2 is vital for immune responses, as lipid rafts facilitate the clustering of signaling molecules, thereby enhancing the efficiency of signal transduction pathways. Raftlin-2 is encoded by a gene located on human chromosome 2q33.1 and mouse chromosome 1 C1.2, with chromosome 2 being the second largest in the human genome, comprising approximately 8% of total DNA and containing over 1,400 genes. Mutations in genes located on this chromosome have been linked to various genetic disorders, highlighting the importance of understanding Raftlin-2′s role in cellular processes and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Raftlin-2 Antibody (F-7) References:
- Alstrom syndrome: confirmation of linkage to chromosome 2p12-13 and phenotypic heterogeneity in three affected sibs. | Zumsteg, U., et al. 2000. J Med Genet. 37: E8. PMID: 10882760
- An ATP-binding cassette gene (ABCG5) from the ABCG (White) gene subfamily maps to human chromosome 2p21 in the region of the Sitosterolemia locus. | Shulenin, S., et al. 2001. Cytogenet Cell Genet. 92: 204-8. PMID: 11435688
- Mutation of ALMS1, a large gene with a tandem repeat encoding 47 amino acids, causes Alström syndrome. | Hearn, T., et al. 2002. Nat Genet. 31: 79-83. PMID: 11941370
- The B cell-specific major raft protein, Raftlin, is necessary for the integrity of lipid raft and BCR signal transduction. | Saeki, K., et al. 2003. EMBO J. 22: 3015-26. PMID: 12805216
- Mutations in ABCA12 underlie the severe congenital skin disease harlequin ichthyosis. | Kelsell, DP., et al. 2005. Am J Hum Genet. 76: 794-803. PMID: 15756637
- A major lipid raft protein raftlin modulates T cell receptor signaling and enhances th17-mediated autoimmune responses. | Saeki, K., et al. 2009. J Immunol. 182: 5929-37. PMID: 19414744
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Raftlin-2 Antibody (F-7) | sc-514540 | 200 µg/ml | $322.00 | |||
Raftlin-2 Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-526376 | 200 µg Ab; 10 µg BP | $361.00 | |||
Raftlin-2 Antibody (F-7) AC | sc-514540 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Raftlin-2 Antibody (F-7) HRP | sc-514540 HRP | 200 µg/ml | $322.00 | |||
Raftlin-2 Antibody (F-7) FITC | sc-514540 FITC | 200 µg/ml | $336.00 | |||
Raftlin-2 Antibody (F-7) PE | sc-514540 PE | 200 µg/ml | $349.00 | |||
Raftlin-2 Antibody (F-7) Alexa Fluor® 488 | sc-514540 AF488 | 200 µg/ml | $364.00 | |||
Raftlin-2 Antibody (F-7) Alexa Fluor® 546 | sc-514540 AF546 | 200 µg/ml | $364.00 | |||
Raftlin-2 Antibody (F-7) Alexa Fluor® 594 | sc-514540 AF594 | 200 µg/ml | $364.00 | |||
Raftlin-2 Antibody (F-7) Alexa Fluor® 647 | sc-514540 AF647 | 200 µg/ml | $364.00 | |||
Raftlin-2 Antibody (F-7) Alexa Fluor® 680 | sc-514540 AF680 | 200 µg/ml | $364.00 | |||
Raftlin-2 Antibody (F-7) Alexa Fluor® 790 | sc-514540 AF790 | 200 µg/ml | $364.00 |