BRAF Antibody (F-3): sc-55522
- BRAF Antibody (F-3) is a mouse monoclonal IgG2a κ, cited in 16 publications, provided at 200 µg/ml
- raised against amino acids 12-156 mapping at the N-terminus of Raf-B of human origin
- Anti-BRAF Antibody (F-3) is recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-BRAF Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for BRAF Antibody (F-3) for WB and IHC(P) applications. These reagents are now offered in bundles with BRAF Antibody (F-3) (see ordering information below).
Raf-B Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Raf-B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Raf-B (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Raf-B, also known as BRAF, plays a crucial role in regulating cell growth and differentiation by acting as a key component in the mitogen-activated protein kinase (MAPK) signaling pathway. This pathway is vital for transmitting signals from cell surface receptors to the nucleus, ultimately influencing cellular responses to growth factors and stress. Raf-B is predominantly located in the cytoplasm, where Raf-B interacts with other signaling molecules, including ERK/MAP kinases and ribosomal S6 kinase, to propagate growth signals. Notably, Raf-B expression is highly restricted, with the highest levels found in the cerebrum and testis, indicating specialized functions in these tissues. Raf-B regulation is critical, as unregulated Raf-B has been associated with oncogenic transformation, making Raf-B a significant target for cancer research and therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRAF Antibody (F-3) | sc-55522 | 200 µg/ml | $322.00 | |||
BRAF Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-525633 | 200 µg Ab; 10 µg BP | $361.00 | |||
BRAF Antibody (F-3): m-IgG2a BP-HRP Bundle | sc-546345 | 200 µg Ab; 10 µg BP | $361.00 | |||
BRAF Antibody (F-3) AC | sc-55522 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BRAF Antibody (F-3) HRP | sc-55522 HRP | 200 µg/ml | $322.00 | |||
BRAF Antibody (F-3) FITC | sc-55522 FITC | 200 µg/ml | $336.00 | |||
BRAF Antibody (F-3) PE | sc-55522 PE | 200 µg/ml | $349.00 | |||
BRAF Antibody (F-3) Alexa Fluor® 488 | sc-55522 AF488 | 200 µg/ml | $364.00 | |||
BRAF Antibody (F-3) Alexa Fluor® 546 | sc-55522 AF546 | 200 µg/ml | $364.00 | |||
BRAF Antibody (F-3) Alexa Fluor® 594 | sc-55522 AF594 | 200 µg/ml | $364.00 | |||
BRAF Antibody (F-3) Alexa Fluor® 647 | sc-55522 AF647 | 200 µg/ml | $364.00 | |||
BRAF Antibody (F-3) Alexa Fluor® 680 | sc-55522 AF680 | 200 µg/ml | $364.00 | |||
BRAF Antibody (F-3) Alexa Fluor® 790 | sc-55522 AF790 | 200 µg/ml | $364.00 |